Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.18 extracted from

  • Bertwistle, D.; Vogt, L.; Aamudalapalli, H.B.; Palmer, D.R.; Sanders, D.A.
    Purification, crystallization and room-temperature X-ray diffraction of inositol dehydrogenase LcIDH2 from Lactobacillus casei BL23 (2014), Acta Crystallogr. Sect. F, 70, 979-983.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene iolG2, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain XL1-Blue Lacticaseibacillus casei

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, microbatch crystallization, mixing of 0.001 ml of 10 mg/ml protein in 25 mM Tris-HCl, pH 8.0, with 0.001 ml of crystallization solution containing 2.8 M ammonium sulfate, 0.18 M citric acid, pH 5.0, room temperature, X-ray diffraction structure determination and analysis at 1.6 A resolution, molecular replacement and model building, the room-temperature radiation tolerance of a protein crystal and its pseudo-translation symmetry, overview Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38330
-
-
Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol + NAD+ Lacticaseibacillus casei
-
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+
-
?
myo-inositol + NAD+ Lacticaseibacillus casei
-
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ i.e. scyllo-inosose ?

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei E1U887 gene iolG1
-
Lacticaseibacillus casei E1U888 gene iolG2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XL1-Blue by ammonium sulfate fractionation, nickel affinity chromatography and dialysis Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol + NAD+
-
Lacticaseibacillus casei 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+
-
?
myo-inositol + NAD+
-
Lacticaseibacillus casei 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ i.e. scyllo-inosose ?

Subunits

Subunits Comment Organism
tetramer 4 * 38330, sequence calculation and crystal structure analysis Lacticaseibacillus casei

Synonyms

Synonyms Comment Organism
inositol dehydrogenase
-
Lacticaseibacillus casei
iolG1
-
Lacticaseibacillus casei
iolG2
-
Lacticaseibacillus casei
LcIDH1
-
Lacticaseibacillus casei
LcIDH2
-
Lacticaseibacillus casei

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Lacticaseibacillus casei

pI Value

Organism Comment pI Value Maximum pI Value
Lacticaseibacillus casei sequence calculation
-
5.33