Cloned (Comment) | Organism |
---|---|
gene iolG2, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain XL1-Blue | Lacticaseibacillus casei |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, microbatch crystallization, mixing of 0.001 ml of 10 mg/ml protein in 25 mM Tris-HCl, pH 8.0, with 0.001 ml of crystallization solution containing 2.8 M ammonium sulfate, 0.18 M citric acid, pH 5.0, room temperature, X-ray diffraction structure determination and analysis at 1.6 A resolution, molecular replacement and model building, the room-temperature radiation tolerance of a protein crystal and its pseudo-translation symmetry, overview | Lacticaseibacillus casei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38330 | - |
- |
Lacticaseibacillus casei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | Lacticaseibacillus casei | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | - |
? | |
myo-inositol + NAD+ | Lacticaseibacillus casei | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | i.e. scyllo-inosose | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | E1U887 | gene iolG1 | - |
Lacticaseibacillus casei | E1U888 | gene iolG2 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XL1-Blue by ammonium sulfate fractionation, nickel affinity chromatography and dialysis | Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | - |
Lacticaseibacillus casei | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | - |
? | |
myo-inositol + NAD+ | - |
Lacticaseibacillus casei | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | i.e. scyllo-inosose | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38330, sequence calculation and crystal structure analysis | Lacticaseibacillus casei |
Synonyms | Comment | Organism |
---|---|---|
inositol dehydrogenase | - |
Lacticaseibacillus casei |
iolG1 | - |
Lacticaseibacillus casei |
iolG2 | - |
Lacticaseibacillus casei |
LcIDH1 | - |
Lacticaseibacillus casei |
LcIDH2 | - |
Lacticaseibacillus casei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lacticaseibacillus casei |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lacticaseibacillus casei | sequence calculation | - |
5.33 |