Cloned (Comment) | Organism |
---|---|
organization of iol genes, three paralogous iol genes GK1897, GK1898, and GK1899 located in tandem within a large gene cluster, determination of the transcription start site, individual expression as His6-tagged proteins in Escherichia coli strain BL21(DE3) | Geobacillus kaustophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | Geobacillus kaustophilus | - |
2,3,4,5,6-pentahydroxycyclohexanone + NADH + H+ | - |
r | |
myo-inositol + NAD+ | Geobacillus kaustophilus PS8 | - |
2,3,4,5,6-pentahydroxycyclohexanone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | - |
three paralogous iol genes located in tandem within a large gene cluster, i.e. GK1897, GK1898, and GK1899 | - |
Geobacillus kaustophilus PS8 | - |
three paralogous iol genes located in tandem within a large gene cluster, i.e. GK1897, GK1898, and GK1899 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Geobacillus kaustophilus strain HTA426 utilizes some inositol stereoisomers, including myo-, D-chiro- and scyllo-inositols, as sole carbon sources. Any of the three isozymes supports myo-inositol-dependent growth. GC-TOF-MS analysis of intracellular inositols in strain HTA426 | Geobacillus kaustophilus | - |
additional information | Geobacillus kaustophilus strain PS8 utilizes myo-inositol as sole carbon source. Any of the three isozymes supports myo-inositol-dependent growth. GC-TOF-MS analysis of intracellular inositols in strain PS8 | Geobacillus kaustophilus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | - |
Geobacillus kaustophilus | 2,3,4,5,6-pentahydroxycyclohexanone + NADH + H+ | - |
r | |
myo-inositol + NAD+ | - |
Geobacillus kaustophilus PS8 | 2,3,4,5,6-pentahydroxycyclohexanone + NADH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Geobacillus kaustophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Geobacillus kaustophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus kaustophilus | |
NADH | - |
Geobacillus kaustophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | Geobacillus kaustophilus HTA426 utilizes some inositol stereoisomers, including myo-, D-chiro- and scyllo-inositols, as sole carbon sources. Any of the three isozymes supports MI-dependent growth | Geobacillus kaustophilus |