Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Bacillus subtilis |
160000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol + NAD+ | Bacillus subtilis | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P26935 | - |
- |
Purification (Comment) | Organism |
---|---|
90% homogeneity, recombinant enzyme | Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Bacillus subtilis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
overepression in Escherichia coli | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + NAD+ | 9% of the activity compared to myo-inositol as substrate | Bacillus subtilis | D-gluconate + NADH | - |
? | |
D-xylose + NAD+ | 7% of the activity compared to myo-inositol as substrate | Bacillus subtilis | ? + NADH | - |
? | |
myo-inositol + NAD+ | - |
Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | can not be replaced by NADP+ | Bacillus subtilis |