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Literature summary for 1.1.1.18 extracted from

  • Fujita, Y.; Shindo, K.; Miwa, Y.; Yoshida, K.
    Bacillus subtiltis inositol dehydrogenase-encoding gene (idh): sequence and expression in Escherichia coli (1991), Gene, 108, 121-125.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE Bacillus subtilis
160000
-
gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol + NAD+ Bacillus subtilis
-
2,4,6/3,5-pentahydroxycyclohexanone + NADH
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P26935
-
-

Purification (Commentary)

Purification (Comment) Organism
90% homogeneity, recombinant enzyme Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Bacillus subtilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
overepression in Escherichia coli Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NAD+ 9% of the activity compared to myo-inositol as substrate Bacillus subtilis D-gluconate + NADH
-
?
D-xylose + NAD+ 7% of the activity compared to myo-inositol as substrate Bacillus subtilis ? + NADH
-
?
myo-inositol + NAD+
-
Bacillus subtilis 2,4,6/3,5-pentahydroxycyclohexanone + NADH
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+ can not be replaced by NADP+ Bacillus subtilis