Literature summary for 1.1.1.178 extracted from

  • Conrad R.S.; Massey L.K.; Sokatch J.R.
    D- and L-leucine metabolism and regulation of their pathways in Pseudomonas putida (1974), J. Bacteriol., 4, 103-111.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
3-hydroxybutyryl-CoA
-
Pseudomonas putida
0.037
-
2-methyl-3-hydroxybutyryl-CoA
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
gel filtration, sucrose density gradient centrifugation Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methyl-3-hydroxybutyryl-CoA + NAD+ Pseudomonas putida isoleucine metabolism 2-methylacetoacetyl-CoA + NADH
-
?
2-methyl-3-hydroxybutyryl-CoA + NAD+ Pseudomonas putida PpG2 isoleucine metabolism 2-methylacetoacetyl-CoA + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
PpG2
-
Pseudomonas putida PpG2
-
PpG2
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.7
-
3-hydroxybutyryl-CoA Pseudomonas putida
16.2
-
2-methyl-3-hydroxybutyryl-CoA Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-3-hydroxybutyryl-CoA + NAD+
-
Pseudomonas putida 2-methylacetoacetyl-CoA + NADH
-
?
2-methyl-3-hydroxybutyryl-CoA + NAD+ isoleucine metabolism Pseudomonas putida 2-methylacetoacetyl-CoA + NADH
-
?
2-methyl-3-hydroxybutyryl-CoA + NAD+
-
Pseudomonas putida PpG2 2-methylacetoacetyl-CoA + NADH
-
?
2-methyl-3-hydroxybutyryl-CoA + NAD+ isoleucine metabolism Pseudomonas putida PpG2 2-methylacetoacetyl-CoA + NADH
-
?
3-hydroxybutyryl-CoA + NAD+
-
Pseudomonas putida acetoacetyl-CoA + NADH
-
?
3-hydroxybutyryl-CoA + NAD+
-
Pseudomonas putida PpG2 acetoacetyl-CoA + NADH
-
?
2-hydroxy-3-methylpentanoyl-CoA + NAD+
-
Pseudomonas putida 3-methyl-2-oxopentanoyl-CoA + NADH
-
?
2-hydroxy-3-methylpentanoyl-CoA + NAD+
-
Pseudomonas putida PpG2 3-methyl-2-oxopentanoyl-CoA + NADH
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ cannot be replaced by NADP+ Pseudomonas putida