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Literature summary for 1.1.1.176 extracted from
- Metabolism of oxo-bile acids and characterization of recombinant 12alpha-hydroxysteroid dehydrogenases from bile acid 7alpha-dehydroxylating human gut bacteria (2018), Appl. Environ. Microbiol., 84, e00235-18 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | [Clostridium] scindens |
| expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | Peptacetobacter hiranonis |
| expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | [Clostridium] hylemonae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01752 | - |
12-oxolithocholic acid | at pH 7.0, temperature not specified in the publication | [Clostridium] scindens |  |
| 0.0327 | - |
12-oxolithocholic acid | at pH 7.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 0.0341 | - |
NADPH | at pH 7.0, temperature not specified in the publication | [Clostridium] scindens | |
| 0.03429 | - |
12-oxolithocholic acid | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
| 0.04037 | - |
NADPH | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
| 0.04831 | - |
NADPH | at pH 7.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 0.07988 | - |
NADP+ | at pH 7.5, temperature not specified in the publication | [Clostridium] scindens | |
| 0.11053 | - |
NADP+ | at pH 8.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 0.12794 | - |
NADP+ | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
| 0.1478 | - |
deoxycholic acid | at pH 8.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 0.17544 | - |
deoxycholic acid | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
| 0.17861 | - |
deoxycholic acid | at pH 7.5, temperature not specified in the publication | [Clostridium] scindens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Peptacetobacter hiranonis | B6FYX7 | - |
- |
| Peptacetobacter hiranonis DSM 13275 | B6FYX7 | - |
- |
| [Clostridium] hylemonae | C0BWQ2 | - |
- |
| [Clostridium] hylemonae DSM 15053 | C0BWQ2 | - |
- |
| [Clostridium] scindens | - |
- |
- |
| [Clostridium] scindens ATCC 35704 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Strep-Tactin resin column chromatography | [Clostridium] scindens |
| Strep-Tactin resin column chromatography | Peptacetobacter hiranonis |
| Strep-Tactin resin column chromatography | [Clostridium] hylemonae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 12-oxochenodeoxycholic acid + NADPH + H+ | 3.65% activity compared to 12-oxolithocholic acid | Peptacetobacter hiranonis | 7-oxodeoxycholic acid + NADP+ | - |
? | |
| 12-oxochenodeoxycholic acid + NADPH + H+ | 4.16% activity compared to 12-oxolithocholic acid | [Clostridium] hylemonae | 7-oxodeoxycholic acid + NADP+ | - |
? | |
| 12-oxochenodeoxycholic acid + NADPH + H+ | 9.55% activity compared to 12-oxolithocholic acid | [Clostridium] scindens | 7-oxodeoxycholic acid + NADP+ | - |
? | |
| 12-oxochenodeoxycholic acid + NADPH + H+ | 9.55% activity compared to 12-oxolithocholic acid | [Clostridium] scindens ATCC 35704 | 7-oxodeoxycholic acid + NADP+ | - |
? | |
| 12-oxochenodeoxycholic acid + NADPH + H+ | 3.65% activity compared to 12-oxolithocholic acid | Peptacetobacter hiranonis DSM 13275 | 7-oxodeoxycholic acid + NADP+ | - |
? | |
| 12-oxochenodeoxycholic acid + NADPH + H+ | 4.16% activity compared to 12-oxolithocholic acid | [Clostridium] hylemonae DSM 15053 | 7-oxodeoxycholic acid + NADP+ | - |
? | |
| 12-oxolithocholic acid + NADPH + H+ | 100% activity | [Clostridium] scindens | deoxycholic acid + NADP+ | - |
? | |
| 12-oxolithocholic acid + NADPH + H+ | 100% activity | Peptacetobacter hiranonis | deoxycholic acid + NADP+ | - |
? | |
| 12-oxolithocholic acid + NADPH + H+ | 100% activity | [Clostridium] hylemonae | deoxycholic acid + NADP+ | - |
? | |
| 12-oxolithocholic acid + NADPH + H+ | 100% activity | [Clostridium] scindens ATCC 35704 | deoxycholic acid + NADP+ | - |
? | |
| 12-oxolithocholic acid + NADPH + H+ | 100% activity | Peptacetobacter hiranonis DSM 13275 | deoxycholic acid + NADP+ | - |
? | |
| 12-oxolithocholic acid + NADPH + H+ | 100% activity | [Clostridium] hylemonae DSM 15053 | deoxycholic acid + NADP+ | - |
? | |
| 3-oxocholic acid + NADPH + H+ | - |
[Clostridium] scindens | cholic acid + NADP+ | - |
r | |
| 3-oxocholic acid + NADPH + H+ | - |
Peptacetobacter hiranonis | cholic acid + NADP+ | - |
r | |
| 3-oxocholic acid + NADPH + H+ | - |
[Clostridium] hylemonae | cholic acid + NADP+ | - |
r | |
| 3-oxocholic acid + NADPH + H+ | - |
[Clostridium] scindens ATCC 35704 | cholic acid + NADP+ | - |
r | |
| 3-oxocholic acid + NADPH + H+ | - |
Peptacetobacter hiranonis DSM 13275 | cholic acid + NADP+ | - |
r | |
| 3-oxocholic acid + NADPH + H+ | - |
[Clostridium] hylemonae DSM 15053 | cholic acid + NADP+ | - |
r | |
| 5beta-cholanic acid-3,12-dione + NADPH + H+ | - |
[Clostridium] scindens | 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3,12-dione + NADPH + H+ | - |
Peptacetobacter hiranonis | 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3,12-dione + NADPH + H+ | - |
[Clostridium] hylemonae | 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3,12-dione + NADPH + H+ | - |
[Clostridium] scindens ATCC 35704 | 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3,12-dione + NADPH + H+ | - |
Peptacetobacter hiranonis DSM 13275 | 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3,12-dione + NADPH + H+ | - |
[Clostridium] hylemonae DSM 15053 | 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3,7,12-trione + NADPH + H+ | - |
[Clostridium] scindens | cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3,7,12-trione + NADPH + H+ | - |
Peptacetobacter hiranonis | cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3,7,12-trione + NADPH + H+ | - |
[Clostridium] hylemonae | cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+ | - |
[Clostridium] scindens | cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+ | - |
Peptacetobacter hiranonis | cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+ | - |
[Clostridium] hylemonae | cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+ | - |
? | |
| 7-oxocholic acid + NADPH + H+ | - |
[Clostridium] scindens | deoxycholic acid + NADP+ | - |
r | |
| 7-oxocholic acid + NADPH + H+ | - |
Peptacetobacter hiranonis | deoxycholic acid + NADP+ | - |
r | |
| cholic acid + NADP+ | 54.44% activity compared to deoxycholic acid | [Clostridium] scindens | 3-oxocholic acid + NADPH + H+ | - |
r | |
| cholic acid + NADP+ | 80.51% activity compared to deoxycholic acid | [Clostridium] hylemonae | 3-oxocholic acid + NADPH + H+ | - |
r | |
| cholic acid + NADP+ | 82.32% activity compared to deoxycholic acid | Peptacetobacter hiranonis | 3-oxocholic acid + NADPH + H+ | - |
r | |
| deoxycholic acid + NADP+ | 100% activity | [Clostridium] scindens | 7-oxocholic acid + NADPH + H+ | - |
r | |
| deoxycholic acid + NADP+ | 100% activity | Peptacetobacter hiranonis | 7-oxocholic acid + NADPH + H+ | - |
r | |
| deoxycholic acid + NADP+ | 100% activity | [Clostridium] hylemonae | 7-oxocholic acid + NADPH + H+ | - |
? | |
| glycodeoxycholic acid + NADP+ | 24.17% activity compared to deoxycholic acid | [Clostridium] hylemonae | ? + NADPH + H+ | - |
r | |
| glycodeoxycholic acid + NADP+ | 25.85% activity compared to deoxycholic acid | Peptacetobacter hiranonis | ? + NADPH + H+ | - |
r | |
| glycodeoxycholic acid + NADP+ | 6.36% activity compared to deoxycholic acid | [Clostridium] scindens | ? + NADPH + H+ | - |
r | |
| additional information | no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3'- or C-7'-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH | Peptacetobacter hiranonis | ? | - |
- |
|
| additional information | no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one and 7-oxocholic acid. No activity with NADH | [Clostridium] hylemonae | ? | - |
- |
|
| additional information | no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH | [Clostridium] scindens | ? | - |
- |
|
| additional information | no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH | [Clostridium] scindens ATCC 35704 | ? | - |
- |
|
| additional information | no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3'- or C-7'-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH | Peptacetobacter hiranonis DSM 13275 | ? | - |
- |
|
| additional information | no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one and 7-oxocholic acid. No activity with NADH | [Clostridium] hylemonae DSM 15053 | ? | - |
- |
|
| taurodeoxycholic acid + NADP+ | 40.33% activity compared to deoxycholic acid | Peptacetobacter hiranonis | ? + NADPH + H+ | - |
r | |
| taurodeoxycholic acid + NADP+ | 45.08% activity compared to deoxycholic acid | [Clostridium] hylemonae | ? + NADPH + H+ | - |
r | |
| taurodeoxycholic acid + NADP+ | 6.05% activity compared to deoxycholic acid | [Clostridium] scindens | ? + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | [Clostridium] hylemonae |
| ? | x * 27300, SDS-PAGE | [Clostridium] scindens |
| ? | x * 28500, SDS-PAGE | Peptacetobacter hiranonis |
| ? | x * 27960, calculated from amino acid sequence | [Clostridium] hylemonae |
| ? | x * 28240, calculated from amino acid sequence | [Clostridium] scindens |
| ? | x * 28490, calculated from amino acid sequence | Peptacetobacter hiranonis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 12alpha-HSDH | - |
[Clostridium] scindens |
| 12alpha-HSDH | - |
Peptacetobacter hiranonis |
| 12alpha-HSDH | - |
[Clostridium] hylemonae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.71 | - |
NADPH | at pH 7.0, temperature not specified in the publication | [Clostridium] scindens | |
| 31.13 | - |
12-oxolithocholic acid | at pH 7.0, temperature not specified in the publication | [Clostridium] scindens | |
| 44.52 | - |
NADPH | at pH 7.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 45.09 | - |
12-oxolithocholic acid | at pH 7.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 66.72 | - |
NADP+ | at pH 8.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 72.09 | - |
12-oxolithocholic acid | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
| 72.52 | - |
deoxycholic acid | at pH 8.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 76.24 | - |
NADPH | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
| 90.74 | - |
NADP+ | at pH 7.5, temperature not specified in the publication | [Clostridium] scindens | |
| 90.93 | - |
deoxycholic acid | at pH 7.5, temperature not specified in the publication | [Clostridium] scindens | |
| 129.75 | - |
NADP+ | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
| 130.62 | - |
deoxycholic acid | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | reductive reaction | Peptacetobacter hiranonis |
| 7 | - |
oxidative reaction | Peptacetobacter hiranonis |
| 7 | - |
reductive reaction | [Clostridium] scindens |
| 7 | - |
reductive reaction | [Clostridium] hylemonae |
| 7.5 | - |
oxidative reaction | [Clostridium] scindens |
| 8 | - |
oxidative reaction | [Clostridium] hylemonae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | dependent on | [Clostridium] scindens | |
| NADPH | dependent on | Peptacetobacter hiranonis | |
| NADPH | dependent on | [Clostridium] hylemonae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 490 | - |
deoxycholic acid | at pH 8.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 510 | - |
deoxycholic acid | at pH 7.5, temperature not specified in the publication | [Clostridium] scindens | |
| 600 | - |
NADP+ | at pH 8.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 740 | - |
deoxycholic acid | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
| 790 | - |
NADPH | at pH 7.0, temperature not specified in the publication | [Clostridium] scindens | |
| 920 | - |
NADPH | at pH 7.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 1010 | - |
NADP+ | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
| 1140 | - |
NADP+ | at pH 7.5, temperature not specified in the publication | [Clostridium] scindens | |
| 1380 | - |
12-oxolithocholic acid | at pH 7.0, temperature not specified in the publication | [Clostridium] hylemonae | |
| 1770 | - |
12-oxolithocholic acid | at pH 7.0, temperature not specified in the publication | [Clostridium] scindens | |
| 1890 | - |
NADPH | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
| 2100 | - |
12-oxolithocholic acid | at pH 7.0, temperature not specified in the publication | Peptacetobacter hiranonis | |
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