Cloned (Comment) | Organism |
---|---|
gene xylB, recombinnat expression in Saccharomyces cerevisiae strain VVT B-67002 | Caulobacter vibrioides |
Protein Variants | Comment | Organism |
---|---|---|
additional information | both D-xylono-gamma-lactone and D-xylonate are produced when the Caulobacter crescentus gene xylB encoding D-xylose dehydrogenase is expressed in Saccharomyces cerevisiae, with or without co-expressionof xylC (D-xylonolactonase). XylC facilitates rapid opening of the lactone and more D-xylonate is initially produced than in its absence. In vivo [1H]NMR analysis of cell extracts, culture media and intact cells is used for analysis. The lactone and linear forms of D-xylonic acid are produced, accumulated intracellularly, and partially exported within 1560 min after D-xylose provision. Co-expression of xylB and xylC leads to rapid loss of pHluorin fluorescence and loss of vitality during production of D-xylonate. Loss of vitality in the presence of D-xylose is correlated to the extracellular pH, but fluorescence is lost from xylB and xylC expressing cells regardless of the extracellular condition. Method optimization, overview | Caulobacter vibrioides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD+ | Caulobacter vibrioides | - |
D-xylonolactone + NADH + H+ | - |
? | |
D-xylose + NAD+ | Caulobacter vibrioides NA1000 | - |
D-xylonolactone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caulobacter vibrioides | B8H1Z0 | - |
- |
Caulobacter vibrioides NA1000 | B8H1Z0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD+ | - |
Caulobacter vibrioides | D-xylonolactone + NADH + H+ | - |
? | |
D-xylose + NAD+ | - |
Caulobacter vibrioides NA1000 | D-xylonolactone + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-xylose 1-dehydrogenase | - |
Caulobacter vibrioides |
XylB | - |
Caulobacter vibrioides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in vivo assay, cell growth at 30°C | Caulobacter vibrioides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Caulobacter vibrioides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Caulobacter vibrioides |