Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.174 extracted from

  • Li, A.; Ilie, A.; Sun, Z.; Lonsdale, R.; Xu, J.; Reetz, M.
    Whole-cell-catalyzed multiple regio- and stereoselective functionalizations in cascade reactions enabled by directed evolution (2016), Angew. Chem. Int. Ed. Engl., 55, 12026-12029.
    View publication on PubMed
Search for more literature for 1.1.1.174

Cloned(Commentary)

Cloned (Comment) Organism
functional recombinant expression in Escherichia coli Priestia megaterium

Protein Variants

Protein Variants Comment Organism
L181F random mutagenesis, A site mutant Priestia megaterium
L181F/I263N random mutagenesis, A site mutant Priestia megaterium
additional information whole-cell-catalyzed multiple regio- and stereoselective functionalizations in cascade reactions enabled by directed evolution: in Escherichia coli, expression of the recombinant enzyme leads to four different mutant whole cells, each leading to one of the three possible stereoisomeric forms of 1,2-cyclohexanediol, namely (R,R)-5, (S,S)-5, (S,R)-5 (meso), or (R,S)-5 (also meso), in a multi-step sequence, overview. Starting from the achiral substrates cyclohexane, cyclohexanol, or cyclohexanone, cyclohexane-1,2-diols as functionalized products are shown to be accessible in three different stereoisomeric forms Priestia megaterium
V78I/A82F/A328F/T438I random mutagenesis, B site mutant Priestia megaterium
V78I/A82I/A328N random mutagenesis, B site mutant Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-cyclohexane-1,2-diol + NAD+ Priestia megaterium
-
 2-hydroxycyclohexan-1-one + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-cyclohexane-1,2-diol + NAD+
-
 Priestia megaterium 2-hydroxycyclohexan-1-one + NADH + H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Priestia megaterium