Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme transcription in Pichia stipitis is induced during growth on L-rhamnose but not on other carbon sources | Scheffersomyces stipitis |
Cloned (Comment) | Organism |
---|---|
gene RHA1, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cereviaie | Scheffersomyces stipitis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | D-glucose suppresses the enzyme | Scheffersomyces stipitis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate specificity, overview | Scheffersomyces stipitis | |
1.5 | - |
L-rhamnose | pH 8.0, 30°C, recombinant enzyme | Scheffersomyces stipitis | |
5 | - |
L-Lyxose | pH 8.0, 30°C, recombinant enzyme | Scheffersomyces stipitis | |
25 | - |
L-Mannose | pH 8.0, 30°C, recombinant enzyme | Scheffersomyces stipitis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, recombinant enzyme, SDS-PAGE | Scheffersomyces stipitis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-rhamnose + NAD+ | Scheffersomyces stipitis | the enzyme is involved in the rhamnose metabolism, overview | L-rhamno-1,4-lactone + NADH | - |
r | |
L-rhamnose + NAD+ | Scheffersomyces stipitis NRC 5568 | the enzyme is involved in the rhamnose metabolism, overview | L-rhamno-1,4-lactone + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | - |
gene RHA1 | - |
Scheffersomyces stipitis NRC 5568 | - |
gene RHA1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme in analytical amounts from Saccharomyces cerevisiae by anion exchange chromatography, and native and SDS-PAGE, tagging of the enzyme strongly affects its activity, overview | Scheffersomyces stipitis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
purified recombinant enzyme | Scheffersomyces stipitis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lyxose + NAD+ | - |
Scheffersomyces stipitis | ? + NADH | - |
? | |
L-lyxose + NAD+ | - |
Scheffersomyces stipitis NRC 5568 | ? + NADH | - |
? | |
L-mannose + NAD+ | - |
Scheffersomyces stipitis | ? + NADH | - |
? | |
L-mannose + NAD+ | - |
Scheffersomyces stipitis NRC 5568 | ? + NADH | - |
? | |
L-rhamnose + NAD+ | the enzyme is involved in the rhamnose metabolism, overview | Scheffersomyces stipitis | L-rhamno-1,4-lactone + NADH | - |
r | |
L-rhamnose + NAD+ | the heterologous protein shows the highest activity and affinity with L-rhamnose and a lower activity and affinity with L-mannose and L-lyxose | Scheffersomyces stipitis | L-rhamno-1,4-lactone + NADH | - |
r | |
L-rhamnose + NAD+ | the enzyme is involved in the rhamnose metabolism, overview | Scheffersomyces stipitis NRC 5568 | L-rhamno-1,4-lactone + NADH | - |
r | |
L-rhamnose + NAD+ | the heterologous protein shows the highest activity and affinity with L-rhamnose and a lower activity and affinity with L-mannose and L-lyxose | Scheffersomyces stipitis NRC 5568 | L-rhamno-1,4-lactone + NADH | - |
r | |
additional information | substrate specificity, overview | Scheffersomyces stipitis | ? | - |
? | |
additional information | substrate specificity, overview | Scheffersomyces stipitis NRC 5568 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, recombinant enzyme, SDS-PAGE | Scheffersomyces stipitis |
Synonyms | Comment | Organism |
---|---|---|
L-rhamnose-1-dehydrogenase | - |
Scheffersomyces stipitis |
NAD-utilizing L-rhamnose 1-dehydrogenase | - |
Scheffersomyces stipitis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Scheffersomyces stipitis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Scheffersomyces stipitis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Scheffersomyces stipitis | |
NAD+ | strictly dependent on | Scheffersomyces stipitis | |
NADH | strictly dependent on | Scheffersomyces stipitis |