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Literature summary for 1.1.1.173 extracted from

  • Koivistoinen, O.M.; Hilditch, S.; Voutilainen, S.P.; Boer, H.; Penttilae, M.; Richard, P.
    Identification in the yeast Pichia stipitis of the first L-rhamnose-1-dehydrogenase gene (2008), FEBS J., 275, 2482-2488.
    View publication on PubMed
Search for more literature for 1.1.1.173

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme transcription in Pichia stipitis is induced during growth on L-rhamnose but not on other carbon sources Scheffersomyces stipitis

Cloned(Commentary)

Cloned (Comment) Organism
gene RHA1, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cereviaie Scheffersomyces stipitis

Inhibitors

Inhibitors Comment Organism Structure
additional information D-glucose suppresses the enzyme Scheffersomyces stipitis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information substrate specificity, overview Scheffersomyces stipitis
1.5
-
 L-rhamnose pH 8.0, 30°C, recombinant enzyme Scheffersomyces stipitis
5
-
 L-Lyxose pH 8.0, 30°C, recombinant enzyme Scheffersomyces stipitis
25
-
 L-Mannose pH 8.0, 30°C, recombinant enzyme Scheffersomyces stipitis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, recombinant enzyme, SDS-PAGE Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-rhamnose + NAD+ Scheffersomyces stipitis the enzyme is involved in the rhamnose metabolism, overview L-rhamno-1,4-lactone + NADH
-
 r
L-rhamnose + NAD+ Scheffersomyces stipitis NRC 5568 the enzyme is involved in the rhamnose metabolism, overview L-rhamno-1,4-lactone + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis
-
 gene RHA1
-
Scheffersomyces stipitis NRC 5568
-
 gene RHA1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme in analytical amounts from Saccharomyces cerevisiae by anion exchange chromatography, and native and SDS-PAGE, tagging of the enzyme strongly affects its activity, overview Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.6
-
 purified recombinant enzyme Scheffersomyces stipitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lyxose + NAD+
-
 Scheffersomyces stipitis ? + NADH
-
 ?
L-lyxose + NAD+
-
 Scheffersomyces stipitis NRC 5568 ? + NADH
-
 ?
L-mannose + NAD+
-
 Scheffersomyces stipitis ? + NADH
-
 ?
L-mannose + NAD+
-
 Scheffersomyces stipitis NRC 5568 ? + NADH
-
 ?
L-rhamnose + NAD+ the enzyme is involved in the rhamnose metabolism, overview Scheffersomyces stipitis L-rhamno-1,4-lactone + NADH
-
 r
L-rhamnose + NAD+ the heterologous protein shows the highest activity and affinity with L-rhamnose and a lower activity and affinity with L-mannose and L-lyxose Scheffersomyces stipitis L-rhamno-1,4-lactone + NADH
-
 r
L-rhamnose + NAD+ the enzyme is involved in the rhamnose metabolism, overview Scheffersomyces stipitis NRC 5568 L-rhamno-1,4-lactone + NADH
-
 r
L-rhamnose + NAD+ the heterologous protein shows the highest activity and affinity with L-rhamnose and a lower activity and affinity with L-mannose and L-lyxose Scheffersomyces stipitis NRC 5568 L-rhamno-1,4-lactone + NADH
-
 r
additional information substrate specificity, overview Scheffersomyces stipitis ?
-
 ?
additional information substrate specificity, overview Scheffersomyces stipitis NRC 5568 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, recombinant enzyme, SDS-PAGE Scheffersomyces stipitis

Synonyms

Synonyms Comment Organism
L-rhamnose-1-dehydrogenase
-
 Scheffersomyces stipitis
NAD-utilizing L-rhamnose 1-dehydrogenase
-
 Scheffersomyces stipitis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Scheffersomyces stipitis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Scheffersomyces stipitis
NAD+ strictly dependent on Scheffersomyces stipitis
NADH strictly dependent on Scheffersomyces stipitis