Crystallization (Comment) | Organism |
---|---|
structure at 1.7 A resolution and molecular docking of inhibition dihydrocelastrol | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
R283S | slight decrease in activity | Staphylococcus aureus |
R287S | strong decrease in activity | Staphylococcus aureus |
R294F | loss of activity | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dihydrocelastrol | competitive inhibitor, presence of dihydrocelastrol effectively reduces bacterial cell viability during host infection | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0886 | - |
D-mannitol 1-phosphate | pH 7.5, temperature not specified in the publication | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | A8YYC5 | - |
- |
Staphylococcus aureus USA300 | A8YYC5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NAD+ | - |
Staphylococcus aureus | D-fructose 6-phosphate + NADH + H+ | - |
? | |
D-mannitol 1-phosphate + NAD+ | - |
Staphylococcus aureus USA300 | D-fructose 6-phosphate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M1PDH | - |
Staphylococcus aureus |
MtlD | - |
Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
dihydrocelastrol | pH 7.5, temperature not specified in the publication | Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
physiological function | M1PDH is essential to endure pH, high salt concentration, and oxidative stress and is required for preventing osmotic burst by regulating pressure potential imposed by mannitol. In a mouse infection model, M1PDH is essential for bacterial survival during infection | Staphylococcus aureus |