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Literature summary for 1.1.1.17 extracted from

  • Nguyen, T.; Kim, T.; Ta, H.; Yeo, W.; Choi, J.; Mizar, P.; Lee, S.; Bae, T.; Chaurasia, A.; Kim, K.
    Targeting mannitol metabolism as an alternative antimicrobial strategy based on the structure-function study of mannitol-1-phosphate dehydrogenase in Staphylococcus aureus (2019), mBio, 10, e02660 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
structure at 1.7 A resolution and molecular docking of inhibition dihydrocelastrol Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
R283S slight decrease in activity Staphylococcus aureus
R287S strong decrease in activity Staphylococcus aureus
R294F loss of activity Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
dihydrocelastrol competitive inhibitor, presence of dihydrocelastrol effectively reduces bacterial cell viability during host infection Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0886
-
D-mannitol 1-phosphate pH 7.5, temperature not specified in the publication Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus A8YYC5
-
-
Staphylococcus aureus USA300 A8YYC5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol 1-phosphate + NAD+
-
Staphylococcus aureus D-fructose 6-phosphate + NADH + H+
-
?
D-mannitol 1-phosphate + NAD+
-
Staphylococcus aureus USA300 D-fructose 6-phosphate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
M1PDH
-
Staphylococcus aureus
MtlD
-
Staphylococcus aureus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.004
-
dihydrocelastrol pH 7.5, temperature not specified in the publication Staphylococcus aureus

General Information

General Information Comment Organism
physiological function M1PDH is essential to endure pH, high salt concentration, and oxidative stress and is required for preventing osmotic burst by regulating pressure potential imposed by mannitol. In a mouse infection model, M1PDH is essential for bacterial survival during infection Staphylococcus aureus