Literature summary for 1.1.1.17 extracted from

Sand, M.; Mingote, A.I.; Santos, H.; Mueller, V.; Averhoff, B.
Mannitol, a compatible solute synthesized by Acinetobacter baylyi in a two-step pathway including a salt-induced and salt-dependent mannitol-1-phosphate dehydrogenase (2013), Environ. Microbiol., 15, 2187-2197.

Search for more literature for 1.1.1.17

Cloned(Commentary)

Cloned (Comment)	Organism
gene mtlD or ACIAD1672, DNA and amino acid sequence determination and analysis, real-time-PCR expression analysis, expression of His-tagged enzyme	Acinetobacter baylyi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
13.6	-	D-fructose 6-phosphate	pH 7.0, 30°C	Acinetobacter baylyi

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	the enzyme is strictly salt-dependent and salt-induced, overview	Acinetobacter baylyi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
81300	-	x * 81300, about, sequence calculation	Acinetobacter baylyi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-fructose 6-phosphate + NADH + H+	Acinetobacter baylyi	-	D-mannitol 1-phosphate + NAD+	-	r
D-fructose 6-phosphate + NADPH + H+	Acinetobacter baylyi	-	D-mannitol 1-phosphate + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter baylyi	-	gene mtlD or ACIAD1672	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged MtlD by nickel affinity chromatography	Acinetobacter baylyi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
130	-	D-fructose 6-phosphate reduction with NADPH, pH 7.0, 30°C	Acinetobacter baylyi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 6-phosphate + NADH + H+	-	Acinetobacter baylyi	D-mannitol 1-phosphate + NAD+	-	r
D-fructose 6-phosphate + NADPH + H+	-	Acinetobacter baylyi	D-mannitol 1-phosphate + NADP+	-	r

Subunits

Subunits	Comment	Organism
?	x * 81300, about, sequence calculation	Acinetobacter baylyi

Synonyms

Synonyms	Comment	Organism
mannitol-1-phosphate dehydrogenase	-	Acinetobacter baylyi
MtlD	-	Acinetobacter baylyi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Acinetobacter baylyi

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	40	70% of maximal activity at 15°C or 35°C, and 50% at 10°C or 40°C	Acinetobacter baylyi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7.5	-	Acinetobacter baylyi

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9	65% and 50% of maximal activity, respectively	Acinetobacter baylyi

Cofactor

Cofactor	Comment	Organism	Structure
additional information	the enzyme prefers NADPH over NADH	Acinetobacter baylyi
NAD+	-	Acinetobacter baylyi
NADH	maximal specific activity with NADH is 15fold lower compared to NADPH	Acinetobacter baylyi
NADPH	preferred cofactor	Acinetobacter baylyi

Expression

Organism	Comment	Expression
Acinetobacter baylyi	the enzyme is salt-dependent and salt-induced, overview	up

General Information

General Information	Comment	Organism
malfunction	deletion of gene mtlD results in a complete loss of salt-dependent mannitol biosynthesis	Acinetobacter baylyi
metabolism	mannitol-1-phosphate dehydrogenase mediates the first step of two-step pathway for osmo-induced synthesis of mannitol, regulated by salinity on the transcriptional as well as on the activity level, overview	Acinetobacter baylyi

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Release 2023.1 (January 2023)