Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | competitive versus NADPH, noncompetitive versus ketopantoate | Escherichia coli | |
Pantoate | noncompetitive versus ketopantoate and NADPH | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADPH | +/-0.0004 | Escherichia coli | |
0.007 | - |
NADP+ | +/-0.002 | Escherichia coli | |
0.12 | - |
ketopantoate | +/-0.008 | Escherichia coli | |
0.26 | - |
Pantoate | +/-0.04 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | does not require Mg2+, independent of divalent metal ions | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
SDS-PAGE, gel filtration | Escherichia coli |
33866 | - |
1 * 33000 gel filtration, 1 * 33866 elelectrospray mass spectrometry analysis, 1 * 33870 amino acid sequence | Escherichia coli |
33870 | - |
1 * 33000 gel filtration, 1 * 33866 elelectrospray mass spectrometry analysis, 1 * 33870 amino acid sequence | Escherichia coli |
34000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ketopantoate + NADPH | Escherichia coli | pantothenate/coenzyme A biosynthetic pathway | D-pantoate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12, expressed in BL21(DE3) | - |
Purification (Comment) | Organism |
---|---|
anion-exchange chromatography, gel filtration chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ketopantoate + NADPH | B-specific | Escherichia coli | D-pantoate + NADP+ | - |
r | |
alpha-ketopantoate + NADPH | pantothenate/coenzyme A biosynthetic pathway | Escherichia coli | D-pantoate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000 gel filtration, 1 * 33866 elelectrospray mass spectrometry analysis, 1 * 33870 amino acid sequence | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |