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Literature summary for 1.1.1.169 extracted from

  • Zheng, R.; Blanchard, J.S.
    Kinetic and mechanistic analysis of the E. coli panE-encoded ketopantoate reductase (2000), Biochemistry, 39, 3708-3717.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
NADP+ competitive versus NADPH, noncompetitive versus ketopantoate Escherichia coli
Pantoate noncompetitive versus ketopantoate and NADPH Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
NADPH +/-0.0004 Escherichia coli
0.007
-
NADP+ +/-0.002 Escherichia coli
0.12
-
ketopantoate +/-0.008 Escherichia coli
0.26
-
Pantoate +/-0.04 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
additional information does not require Mg2+, independent of divalent metal ions Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
SDS-PAGE, gel filtration Escherichia coli
33866
-
1 * 33000 gel filtration, 1 * 33866 elelectrospray mass spectrometry analysis, 1 * 33870 amino acid sequence Escherichia coli
33870
-
1 * 33000 gel filtration, 1 * 33866 elelectrospray mass spectrometry analysis, 1 * 33870 amino acid sequence Escherichia coli
34000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-ketopantoate + NADPH Escherichia coli pantothenate/coenzyme A biosynthetic pathway D-pantoate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K12, expressed in BL21(DE3)
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange chromatography, gel filtration chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ketopantoate + NADPH B-specific Escherichia coli D-pantoate + NADP+
-
r
alpha-ketopantoate + NADPH pantothenate/coenzyme A biosynthetic pathway Escherichia coli D-pantoate + NADP+
-
r

Subunits

Subunits Comment Organism
monomer 1 * 33000 gel filtration, 1 * 33866 elelectrospray mass spectrometry analysis, 1 * 33870 amino acid sequence Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Escherichia coli
NADPH
-
Escherichia coli