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Literature summary for 1.1.1.168 extracted from
- Discovery of a new NADPH-dependent aldo-keto reductase from Candida orthopsilosis catalyzing the stereospecific synthesis of (R)-pantolactone by genome mining (2019), J. Biotechnol., 291, 26-34 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Candida orthopsilosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 1 mM | Candida orthopsilosis |  |
| Co2+ | 81.9% residual activity at 1 mM | Candida orthopsilosis | |
| Cu2+ | 67.8% residual activity at 1 mM | Candida orthopsilosis | |
| EDTA | - |
Candida orthopsilosis | |
| Fe2+ | 81% residual activity at 1 mM | Candida orthopsilosis | |
| Mg2+ | 80.2% residual activity at 1 mM | Candida orthopsilosis | |
| Mn2+ | 67.6% residual activity at 1 mM | Candida orthopsilosis | |
| Pb2+ | complete inhibition at 1 mM | Candida orthopsilosis | |
| Zn2+ | 71.7% residual activity at 1 mM | Candida orthopsilosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
2-dehydropantolactone | at pH 7.0 and 30°C | Candida orthopsilosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 1 mM Ni2+ and Ca2+ have almost no influence over the enzyme activity | Candida orthopsilosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-dehydropantolactone + NADPH + H+ | Candida orthopsilosis | - |
(R)-pantolactone + NADP+ | - |
? | |
| 2-dehydropantolactone + NADPH + H+ | Candida orthopsilosis 90-125 | - |
(R)-pantolactone + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida orthopsilosis | H8XA83 | - |
- |
| Candida orthopsilosis 90-125 | H8XA83 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Candida orthopsilosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-dehydropantolactone + NADPH + H+ | - |
Candida orthopsilosis | (R)-pantolactone + NADP+ | - |
? | |
| 2-dehydropantolactone + NADPH + H+ | 100% activity, 99% conversion and more than 99% enantiomeric excess within 5 h | Candida orthopsilosis | (R)-pantolactone + NADP+ | - |
? | |
| 2-dehydropantolactone + NADPH + H+ | - |
Candida orthopsilosis 90-125 | (R)-pantolactone + NADP+ | - |
? | |
| 2-dehydropantolactone + NADPH + H+ | 100% activity, 99% conversion and more than 99% enantiomeric excess within 5 h | Candida orthopsilosis 90-125 | (R)-pantolactone + NADP+ | - |
? | |
| ethyl 2-oxopropanoate + NADPH + H+ | 0.496% activity compared to 2-dehydropantolactone | Candida orthopsilosis | ethyl (2R)-2-hydroxypropanoate + NADP+ | - |
? | |
| ethyl 2-oxopropanoate + NADPH + H+ | 0.496% activity compared to 2-dehydropantolactone | Candida orthopsilosis 90-125 | ethyl (2R)-2-hydroxypropanoate + NADP+ | - |
? | |
| ethyl oxo(phenyl)acetate + NADPH + H+ | 3.45% activity compared to 2-dehydropantolactone | Candida orthopsilosis | ethyl (2R)-hydroxy(phenyl)acetate + NADP+ | - |
? | |
| ethyl oxo(phenyl)acetate + NADPH + H+ | 3.45% activity compared to 2-dehydropantolactone | Candida orthopsilosis 90-125 | ethyl (2R)-hydroxy(phenyl)acetate + NADP+ | - |
? | |
| methyl 2-oxopropanoate + NADPH + H+ | 0.501% activity compared to 2-dehydropantolactone | Candida orthopsilosis | methyl (2R)-2-hydroxypropanoate + NADP+ | - |
? | |
| methyl oxo(phenyl)acetate + NADPH + H+ | 3.94% activity compared to 2-dehydropantolactone | Candida orthopsilosis | methyl (2R)-hydroxy(phenyl)acetate + NADP+ | - |
? | |
| additional information | the enzyme does not reduce 4-nitrobenzaldehyde | Candida orthopsilosis | ? | - |
- |
|
| additional information | the enzyme does not reduce 4-nitrobenzaldehyde | Candida orthopsilosis 90-125 | ? | - |
- |
|
| pyridine 3-aldehyde + NADPH + H+ | 0.538% activity compared to 2-dehydropantolactone | Candida orthopsilosis | (pyridin-3-yl)methanol + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, His6-tagged enzyme, SDS-PAGE | Candida orthopsilosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AKR | - |
Candida orthopsilosis |
| NADPH-dependent aldo-keto reductase | - |
Candida orthopsilosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Candida orthopsilosis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | the activity remains above 50% of the maximum activity between 30 and 55°C | Candida orthopsilosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | the recombinant enzyme exhibits high thermal stability with half-lives of 6.3 h, 3.7 h and 52 min at 40, 45 and 50°C, respectively | Candida orthopsilosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Candida orthopsilosis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | more than 40% activity between pH 6.0 and 8.0 | Candida orthopsilosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Candida orthopsilosis | |
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