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Literature summary for 1.1.1.159 extracted from
- A novel 7alpha-hydroxysteroid dehydrogenase Magnesium ion significantly enhances its activity and thermostability (2021), Int. J. Biol. Macromol., 177, 111-118 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | unidentified microorganism |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | magnesium ion significantly enhances activity and thermostability of the enzyme. The enzymatic activity increases 40fold in the presence of 50 mM Mg2+, and T0.5 increases by approximately 6°C | unidentified microorganism |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| unidentified microorganism | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20 | - |
with ethyl benzoylacetate as substrate, at pH 10.5, temperature not specified in the publication | unidentified microorganism |
| 188.3 | - |
with taurochenodeoxycholic acid as substrate, at pH 10.5, temperature not specified in the publication | unidentified microorganism |
| 217.6 | - |
with glycochenodeoxycholic acid as substrate, at pH 10.5, temperature not specified in the publication | unidentified microorganism |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl benzoylacetate + NAD(P)+ | - |
unidentified microorganism | ? | - |
? | |
| glycochenodeoxycholic acid + NAD(P)+ | - |
unidentified microorganism | ? | - |
? | |
| taurochenodeoxycholic acid + NAD(P)+ | - |
unidentified microorganism | tauroursodeoxycholic acid + NAD(P)H + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 7alpha-HSDH | - |
unidentified microorganism |
| J-1-1 | - |
unidentified microorganism |
| NADP(H)-dependent 7alpha-HSDH | - |
unidentified microorganism |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
73% of the enzyme activity is maintained after heat treatment at 40°C for 100 h | unidentified microorganism |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
unidentified microorganism | |
| NADP+ | - |
unidentified microorganism | |
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Release 2023.1 (January 2023)
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