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Literature summary for 1.1.1.159 extracted from
- Functional contribution of coenzyme specificity-determining sites of 7alpha-hydroxysteroid dehydrogenase from Clostridium absonum (2017), Comput. Biol. Chem., 70, 89-95 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Clostridium sardiniense |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R16A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Clostridium sardiniense |
| R16A/R194A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Clostridium sardiniense |
| R194A | mutant with increased catalytic efficiency toward NADP+, as compared to the wild type enzyme | Clostridium sardiniense |
| R38A | inactive | Clostridium sardiniense |
| R38A/R194A | inactive | Clostridium sardiniense |
| T15A | the catalytic efficiency of the mutant decreases by more than 4.5times, compared to the wild type and the affinity drops dramatically (Km value increases by nearly 17times) | Clostridium sardiniense |
| T15A/R16A/R194A | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Clostridium sardiniense |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.69 | - |
NADP+ | wild type enzyme, pH and temperature not specified in the publication | Clostridium sardiniense |  |
| 0.96 | - |
NADP+ | mutant enzyme R16A/R194A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 2.95 | - |
NADP+ | mutant enzyme T15A/R16A/R194A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 4.25 | - |
NADP+ | mutant enzyme R16A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 4.71 | - |
NADP+ | mutant enzyme R194A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 12.33 | - |
NADP+ | mutant enzyme T15A, pH and temperature not specified in the publication | Clostridium sardiniense | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium sardiniense | G9FRD7 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| taurochenodeoxycholic + NADP+ | - |
Clostridium sardiniense | ? + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 7alpha-HSDH | - |
Clostridium sardiniense |
| NADP(H)-dependent 7alpha-hydroxysteroid dehydrogenase | - |
Clostridium sardiniense |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.23 | - |
NADP+ | mutant enzyme T15A/R16A/R194A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 3.86 | - |
NADP+ | mutant enzyme R16A/R194A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 4.89 | - |
NADP+ | wild type enzyme, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 15.85 | - |
NADP+ | mutant enzyme T15A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 25.26 | - |
NADP+ | mutant enzyme R16A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 111.7 | - |
NADP+ | mutant enzyme R194A, pH and temperature not specified in the publication | Clostridium sardiniense | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Clostridium sardiniense | |
| NADPH | - |
Clostridium sardiniense | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.09 | - |
NADP+ | mutant enzyme T15A/R16A/R194A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 1.29 | - |
NADP+ | mutant enzyme T15A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 4.02 | - |
NADP+ | mutant enzyme R16A/R194A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 5.95 | - |
NADP+ | mutant enzyme R16A, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 7.1 | - |
NADP+ | wild type enzyme, pH and temperature not specified in the publication | Clostridium sardiniense | |
| 23.72 | - |
NADP+ | mutant enzyme R194A, pH and temperature not specified in the publication | Clostridium sardiniense | |
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