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Literature summary for 1.1.1.156 extracted from
- Purification and properties of two different dihydroxyacetone reductases in Gluconobacter suboxydans grown on glycerol (2008), Biosci. Biotechnol. Biochem., 72, 2124-2132.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
dihydroxyacetone | at pH 6.5, in 50 mM potassium phosphate buffer, at 25°C | Gluconobacter oxydans |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Gluconobacter oxydans | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
SDS-PAGE | Gluconobacter oxydans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
| Gluconobacter oxydans IFO 3255 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography, hydroxyapatite column chromatography, BD Sepharose column chromatography, and DEAE-Sephadex A-50 gel filtration | Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.06 | - |
crude extract, at 25°C | Gluconobacter oxydans |
| 13.71 | - |
after 228.5fold purification, at 25°C | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroxyacetone + NADH + H+ | less than 10% of the enzyme activity of NADPH-DHAR with NADPH is observed with NADH | Gluconobacter oxydans | glycerol + NAD+ | - |
r | |
| dihydroxyacetone + NADH + H+ | less than 10% of the enzyme activity of NADPH-DHAR with NADPH is observed with NADH | Gluconobacter oxydans IFO 3255 | glycerol + NAD+ | - |
r | |
| dihydroxyacetone + NADPH + H+ | - |
Gluconobacter oxydans | glycerol + NADP+ | - |
r | |
| dihydroxyacetone + NADPH + H+ | - |
Gluconobacter oxydans IFO 3255 | glycerol + NADP+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dihydroxyacetone reductase | - |
Gluconobacter oxydans |
| glycerol:NADP 2-oxidoreductase, dihydroxyacetone forming | - |
Gluconobacter oxydans |
| NADP-dependent GLDH | - |
Gluconobacter oxydans |
| NADPH-DHAR | - |
Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | dependent on | Gluconobacter oxydans | |
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Release 2023.1 (January 2023)
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