Literature summary for 1.1.1.153 extracted from

Supangat, S.; Seo, K.H.; Choi, Y.K.; Park, Y.S.; Son, D.; Han, C.D.; Lee, K.H.
Structure of Chlorobium tepidum sepiapterin reductase complex reveals the novel substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin (2006), J. Biol. Chem., 281, 2249-2256.

Search for more literature for 1.1.1.153

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified native and selenomethionine-labeled enzyme alone and in complex with NADP+ and sepiapterin, hanging drop vapour diffusion method, 18°C, 0.004 ml protein solution, with or without 10 mM NADP+ and sepiapterin, is mixed with 0.001 ml of a mixture of 0.004 ml or reservoir solution containing 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, and 34% PEG 400, and 0.001 ml additive solution 1 M guanidinium hydrochloride, X-ray diffraction structure determination and analysis at 2.1 A and 1.7 A resolution, respectively	Chlorobaculum tepidum

Crystallization (Comment)

Organism

purified native and selenomethionine-labeled enzyme alone and in complex with NADP+ and sepiapterin, hanging drop vapour diffusion method, 18°C, 0.004 ml protein solution, with or without 10 mM NADP+ and sepiapterin, is mixed with 0.001 ml of a mixture of 0.004 ml or reservoir solution containing 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, and 34% PEG 400, and 0.001 ml additive solution 1 M guanidinium hydrochloride, X-ray diffraction structure determination and analysis at 2.1 A and 1.7 A resolution, respectively

Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+	Chlorobaculum tepidum	last step of the tetrahydrobiopterin biosynthesis	L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Chlorobaculum tepidum	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+	active site structure, substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin, stereospecific hydride transfer, mechanism, structure-function relationship, overview	Chlorobaculum tepidum	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+	last step of the tetrahydrobiopterin biosynthesis	Chlorobaculum tepidum	L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+	-	?
6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+	substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin, structure-function relationship	Chlorobaculum tepidum	L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+	-	?

Subunits

Subunits	Comment	Organism
additional information	structure-function relationship, amino acid sequence comparison of several species	Chlorobaculum tepidum

Synonyms

Synonyms	Comment	Organism
SR	-	Chlorobaculum tepidum

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	determination and analysis of the binding structure of NADPH and NADP+ to the enzyme, overview	Chlorobaculum tepidum