Any feedback?
Literature summary for 1.1.1.153 extracted from
- On the role of sepiapterin reductase in the biosynthesis of tetrahydrobiopterin (1987), Arch. Biochem. Biophys., 255, 254-266.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inhibition by specific antibody from rabbits | Rattus norvegicus | |
| N-acetylserotonin | - |
Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Rattus norvegicus | |
| 0.002 | - |
pyruvoyltetrahydropterin | pH 7.4, 37°C | Rattus norvegicus | |
| 0.007 | - |
1'-hydroxy-2'-oxopropyltetrahydropterin | pH 7.4, 37°C | Rattus norvegicus | |
| 0.008 | - |
lactoyltetrahydropterin | pH 7.4, 37°C | Rattus norvegicus | |
| 0.009 | - |
sepiapterin | pH 7.4, 37°C | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27500 | - |
2 * 27500, SDS-PAGE | Rattus norvegicus |
| 56000 | - |
second band in SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-(S)-lactoyl-7,8-dihydropterin + NADPH | Rattus norvegicus | terminal step in the biosynthetic pathway for tetrahydrobiopterin | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
| 6-(S)-lactoyl-7,8-dihydropterin + NADPH | Rattus norvegicus | catalyzes the complete reduction of pyrovoyltetrahydropterin to tetrahydrobiopterin | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
| Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
| native enzyme from erythrocytes by hydroxylapatite and NADP+-affinity chromatography, to homogeneity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Rattus norvegicus | - |
| blood | - |
Rattus norvegicus | - |
| brain | - |
Rattus norvegicus | - |
| erythrocyte | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
| thymus | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1'-hydroxy-2'-oxopropyltetrahydropterin + NADP+ | - |
Rattus norvegicus | L-erythro-tetrahydrobiopterin + NADPH | - |
? | |
| 1'-hydroxy-2'-oxopropyltetrahydropterin + NADPH | - |
Rattus norvegicus | L-erythrotetrahydrobiopterin + NADP+ | - |
? | |
| 6-(S)-lactoyl-7,8-dihydropterin + NADPH | - |
Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
| 6-(S)-lactoyl-7,8-dihydropterin + NADPH | terminal step in the biosynthetic pathway for tetrahydrobiopterin | Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
| 6-(S)-lactoyl-7,8-dihydropterin + NADPH | catalyzes the complete reduction of pyrovoyltetrahydropterin to tetrahydrobiopterin | Rattus norvegicus | 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+ | - |
? | |
| 6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ | - |
Rattus norvegicus | L-erythro-tetrahydrobiopterin + 2 NADP+ | - |
? | |
| lactoyltetrahydropterin + NADPH | - |
Rattus norvegicus | L-erythrotetrahydrobiopterin + NADP+ | - |
? | |
| lactoyltetrahydropterin + NADPH + H+ | - |
Rattus norvegicus | L-erythro-tetrahydrobiopterin + NADP+ | - |
? | |
| additional information | descending substrate preference: sepiapterin, lactoyltetrahydropterin, pyruvoyltetrahydropterin, 1'-hydroxy-2'-oxopropyltetrahydropterin | Rattus norvegicus | ? | - |
? | |
| pyruvoyltetrahydropterin + NADPH | - |
Rattus norvegicus | L-erythrotetrahydrobiopterin + NADP+ | - |
? | |
| sepiapterin + NADPH + H+ | best substrate | Rattus norvegicus | dihydrobiopterin + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Rattus norvegicus |
| homodimer | 2 * 27500, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
