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Literature summary for 1.1.1.149 extracted from

  • Robertson, W.R.; Frost, J.; Hoyer, P.E.; Weinkove, C.
    20alpha-Hydroxysteroid dehydrogenase activity in the rat corpus luteum; a quantitative cytochemical study (1982), J. Steroid Biochem., 17, 237-243.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Dimethyl formamide 5-10% Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
17alpha-hydroxyprogesterone most inhibitory at pH 8.0, significant at pH 6.8 Rattus norvegicus
20beta-hydroxypregn-4-en-3-one marginally inhibitory at pH 8.0 Rattus norvegicus
androstendione most inhibitory at pH 8.0 Rattus norvegicus
phenazine methosulfate 0.01-0.3 mg/ml Rattus norvegicus
progesterone 1.5 mM marginally inhibitory at pH 8.0, 0.75-1.5 mM reduces activity at pH 6.8 by 46 and 60%, respectively; most inhibitory at pH 8.0, significant at pH 6.8 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NADP+ 0.01-0.1 mM, 6 min Rattus norvegicus
0.3
-
20alpha-hydroxyprogesterone 0.015-0.1 mM, 6 min Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
adult, female, Sprague-Dawley
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information unfixed tissue section, soluble, retention by PVA Rattus norvegicus
-
ovary
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with 20beta isomer of 20alpha-hydroxyprogesterone, rising activity with 20alpha-hydroxyprogesterone concentrations over the range 0.05-1.0 mM, increasing activity with NADP+ over the range 0.01-0.2 mM, no NAD+ activity over this concentration range Rattus norvegicus ?
-
?
progesterone + NADPH
-
Rattus norvegicus 20alpha-hydroxyprogesterone + NADP+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
30 min Rattus norvegicus
37
-
6 min Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.2
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 pH 6.8 favouring progesterone to 20alpha-hydroxyprogesterone reaction and pH 8.0 vice versa Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
additional information nitroblue tetrazolium as hydrogen acceptor Rattus norvegicus
NADP+
-
Rattus norvegicus