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Literature summary for 1.1.1.149 extracted from
- Purification and characterization of rat ovarian 20alpha-hydroxysteroid dehydrogenase (1991), Biochim. Biophys. Acta, 1079, 112-118.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| isozyme HSD1, HSD2 | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00087 | - |
NADP+ | HSD2 | Rattus norvegicus |  |
| 0.00105 | - |
NADP+ | HSD1 | Rattus norvegicus | |
| 0.00475 | - |
20alpha-hydroxy-4-pregnen-3-one | HSD1 | Rattus norvegicus | |
| 0.00516 | - |
20alpha-hydroxy-4-pregnen-3-one | HSD2 | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
1 * 33000, SDS-PAGE | Rattus norvegicus |
| 41000 | - |
gel filtration, non-reducing conditions | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
female, Wistar-Imamichi strain | - |
| Rattus norvegicus Wistar-Imamichi | - |
female, Wistar-Imamichi strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isozyme HSD1 and HSD2, anion-exchange and affinity chromatography, capillary electrophoresis | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ovary | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2769 | - |
HSD2, reduction of 17alpha-hydroxyprogesterone | Rattus norvegicus |
| 1.462 | - |
HSD1, reduction of 17alpha-hydroxyprogesterone | Rattus norvegicus |
| 3.815 | - |
HSD2, reduction of progesterone | Rattus norvegicus |
| 4.618 | - |
HSD1 | Rattus norvegicus |
| 6.515 | - |
HSD2 | Rattus norvegicus |
| 8.154 | - |
HSD1, reduction of progesterone | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17alpha-hydroxyprogesterone + NADPH | - |
Rattus norvegicus | 17alpha,20alpha-dihydroxyprogesterone + NADP+ | - |
? | |
| 17alpha-hydroxyprogesterone + NADPH | - |
Rattus norvegicus Wistar-Imamichi | 17alpha,20alpha-dihydroxyprogesterone + NADP+ | - |
? | |
| additional information | no activity with corticosterone and 5alpha-dihydrotestosterone as substrates, other substrate: 17alpha-hydroxyprogesterone | Rattus norvegicus | ? | - |
? | |
| additional information | no activity with corticosterone and 5alpha-dihydrotestosterone as substrates, other substrate: 17alpha-hydroxyprogesterone | Rattus norvegicus Wistar-Imamichi | ? | - |
? | |
| progesterone + NADPH | - |
Rattus norvegicus | 20alpha-hydroxyprogesterone + NADP+ | - |
r | |
| progesterone + NADPH | - |
Rattus norvegicus Wistar-Imamichi | 20alpha-hydroxyprogesterone + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000, SDS-PAGE | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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