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Literature summary for 1.1.1.148 extracted from

  • Bellemare, V.; Faucher, F.; Breton, R.; Luu-The, V.
    Characterization of 17alpha-hydroxysteroid dehydrogenase activity (17alpha-HSD) and its involvement in the biosynthesis of epitestosterone (2005), BMC Biochem., 6, 12.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cDNA cloning, subcloning in Escherichia coli, expression as GST-fusion protein in Escherichia coli strain BL21(DE3), stable expression in HEK-293 cells Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q91WR5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney high expression level Mus musculus
-
additional information enzyme tissue distribution in male and female mice, overview Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-dihydrotestosterone + NADPH
-
Mus musculus 5alpha-androstane-3alpha,17alpha-diol + NADP+
-
?
5alpha-pregnane-3,20-dione + NADPH + H+
-
Mus musculus 3alpha,5alpha-3-hydroxypregnan-20-one + NADP+
-
?
androstanedione + NADPH
-
Mus musculus 5alpha-androstane-3-one-17alpha-ol + NADP+
-
?
androstenedione + NADPH + H+
-
Mus musculus epitestosterone + NADP+
-
?
androsterone + NADPH
-
Mus musculus 5alpha-androstane-3alpha,17alpha-diol + NADP+
-
?
dehydroepiandrosterone + NADPH
-
Mus musculus 5-androstene-3alpha,17alpha-diol + NADP+
-
?
additional information the enzyme is involved in the biosynthesis of epitestosterone, the 17alpha-epimer of testosterone Mus musculus ?
-
?
additional information substrate specificity of the recombinant enzyme, overview Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
17alpha-HSD
-
Mus musculus
17alpha-hydroxysteroid dehydrogenase
-
Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Mus musculus