Cloned (Comment) | Organism |
---|---|
expression of His6-tagged isozyme 11beta-HSD1 in Escherichia coli strain BL21(DE3) | Cavia porcellus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant isozyme 11beta-HSD1 in complex with substrate, cofactor and analogue, or inhibitor, 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 2mM Tris(carboxyethyl)phosphine, 5% glycerol, 0.5 M guanidinum hydrochloride, 0.05% Anapoe X-100, 0.02 mM cortisone, and 0.04 mM NADP+ analogue 3-aminopyridine adenine dinucleotide phosphate AADP, NADP+, BVT-4584, or cortisone, hanging drop vapour diffusion method, mixing with equal volume of precipitation solution containing 38-40% PEG 550 mono methyl ether, and 0.1 M Bis-Tris, pH 6.5, equlibration against 1 ml precipitation solution, 5-6 days, X-ray diffraction structure determination and analysis at 2.5 A resolution | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BVT-4584 | - |
Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | the active site of isozyme 11beta-HSD1 is located on the lumen side | Cavia porcellus | 5783 | - |
endoplasmic reticulum membrane | - |
Cavia porcellus | 5789 | - |
additional information | modeling of enzyme-bilayer interaction structure | Cavia porcellus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisone + NADPH + H+ | Cavia porcellus | isozyme 11beta-HSD1 catalyzes the forward reaction with cofactor NADPH | cortisol + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | Q6QLL4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged isozyme 11beta-HSD1 from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cortisone + NADPH + H+ | isozyme 11beta-HSD1 catalyzes the forward reaction with cofactor NADPH | Cavia porcellus | cortisol + NADP+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
11beta-HSD1 | - |
Cavia porcellus |
11beta-hydroxysteroid dehydrogenase type 1 | - |
Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Cavia porcellus |