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Literature summary for 1.1.1.145 extracted from

  • Zhu, Q.; Pan, P.; Chen, X.; Wang, Y.; Zhang, S.; Mo, J.; Li, X.; Ge, R.S.
    Human placental 3beta-hydroxysteroid dehydrogenase/steroid ?5,4-isomerase 1 Identity, regulation and environmental inhibitors (2019), Toxicology, 425, 152253 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C83A mutation produces fully active enzyme with elevated Km values for ligands Homo sapiens
C83S mutation in the active site abolishes the dehydrogenase and isomerase activities of the enzyme Homo sapiens
D257I complete loss of dehydrogenase and isomerase activities Homo sapiens
D258I complete loss of dehydrogenase and isomerase activities Homo sapiens
D35A/K36R double mutations cause conversion of the cofactor dependence from NAD+ to NADP+ Homo sapiens
K158Q complete loss of dehydrogenase activity Homo sapiens
S124A complete loss of dehydrogenase activity Homo sapiens
Y154F complete loss of dehydrogenase activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(+)-gossypol potent inhibitor, competitive Homo sapiens
(-)-gossypol weak inhibitor, competitive Homo sapiens
bis (2-butoxyethyl) phthalate moderate inhibitor, competitive Homo sapiens
di-heptyl phthalate potent inhibitor, competitive Homo sapiens
di-n-butyl phthalate potent inhibitor, competitive Homo sapiens
di-n-octyl phthalate weak inhibitor Homo sapiens
dicyclohexyl phthalate moderate inhibitor, competitive Homo sapiens
HPTE potent inhibitor, competitive Homo sapiens
methoxychlor insecticide, potent inhibitor, competitive Homo sapiens
organotin can inhibit placental HSD3B1 activity, thus blocking the formation of progesterone and disrupting pregnancy Homo sapiens
tributyltin moderate inhibitor, competitive Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-
smooth endoplasmic reticulum membrane-bound enzyme Homo sapiens 5790
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P14060
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland
-
Homo sapiens
-
placenta intermediate trophoblast and syncytiotrophoblasts Homo sapiens
-
prostate gland
-
Homo sapiens
-
skin
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydroepiandrosterone + NAD+
-
Homo sapiens androstenedione + NADH + H+
-
?
pregnenolone + NAD+
-
Homo sapiens progesterone + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
3beta-hydroxysteroid dehydrogenase/steroid DELTA5,4-isomerase
-
Homo sapiens
HSD3B1
-
Homo sapiens

Expression

Organism Comment Expression
Homo sapiens GATA3 negatively regulates the expression of HSD3B1 down
Homo sapiens expression is regulated by GATA2 and TEF-5 transcription factors. GATA2 is the positively regulatory transcription factor up

General Information

General Information Comment Organism
metabolism HSD3B1 is a unique enzyme for progesterone synthesis and is also involved in the estrogen production in human placenta Homo sapiens