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Literature summary for 1.1.1.145 extracted from

  • Lee, H.J.; Nakayasu, M.; Akiyama, R.; Kobayashi, M.; Miyachi, H.; Sugimoto, Y.; Umemoto, N.; Saito, K.; Muranaka, T.; Mizutani, M.
    Identification of a 3beta-hydroxysteroid dehydrogenase/ 3-ketosteroid reductase involved in alpha-tomatine biosynthesis in tomato (2019), Plant Cell Physiol., 60, 1304-1315 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the enzyme (Sl3bHSD1) in potato hairy roots Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02402
-
 dehydrotomatidine pH 7.2, 30°C Solanum lycopersicum
0.04534
-
 pregnenolone pH 7.2, 30°C Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower high expression Solanum lycopersicum
-
fruit high expression Solanum lycopersicum
-
leaf high expression Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydrotomatidine + NADH + H+
-
 Solanum lycopersicum tomatid-4-en-3-one + NAD+
-
 ?
pregnenolone + NAD+
-
 Solanum lycopersicum progesterone + NADH + H+
-
 ?
tomatid-3-one + NAD+
-
 Solanum lycopersicum tomatidine + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
3beta-hydroxysteroid dehydrogenase/3-ketosteroid reductase
-
 Solanum lycopersicum
Sl3betaHSD1
-
 Solanum lycopersicum
Solyc01g73640
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1145.21
-
 pregnenolone pH 7.2, 30°C Solanum lycopersicum
2769.17
-
 dehydrotomatidine pH 7.2, 30°C Solanum lycopersicum

General Information

General Information Comment Organism
metabolism key enzyme involved in the conversion processes from dehydrotomatidine to tomatidine. The enzyme is involved in alpha-tomatine biosynthesis. alpha-Tomatine and dehydrotomatine are major steroidal glycoalkaloids that accumulate in the mature green fruits, leaves and flowers of tomato (Solanum lycopersicum), and function as defensive compounds against bacteria, fungi, insects and animals Solanum lycopersicum