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Literature summary for 1.1.1.145 extracted from

  • Herl, V.; Frankenstein, J.; Meitinger, N.; Mueller-Uri, F.; Kreis, W.
    Delta5-3beta-hydroxysteroid dehydrogenase (3betaHSD) from Digitalis lanata. Heterologous expression and characterisation of the recombinant enzyme (2007), Planta Med., 73, 704-710.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, His-tag Digitalis lanata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
pregnenolone pH 7.8, 30°C Digitalis lanata
0.106
-
NADH pH 7.8, 30°C Digitalis lanata
0.116
-
5alpha-pregnane-3beta-ol-20-one pH 7.8, 30°C Digitalis lanata
0.196
-
5alpha-pregnane-3,20-dione pH 7.8, 30°C Digitalis lanata
0.26
-
NAD+ pH 7.8, 30°C Digitalis lanata
0.314
-
5beta-pregnane-3beta-ol-20-one pH 7.8, 30°C Digitalis lanata
0.642
-
5-androsten-3beta-ol-17-one pH 7.8, 30°C Digitalis lanata
1.725
-
5beta-pregnane-3,20-dione pH 7.8, 30°C Digitalis lanata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28561
-
x * 28561, calculated Digitalis lanata

Organism

Organism UniProt Comment Textmining
Digitalis lanata Q93Y47
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Digitalis lanata

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Digitalis lanata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-androsten-17beta-ol-3-one + NADH + H+
-
Digitalis lanata 4-androsten-3beta,17beta-diol + NAD+
-
r
5-androsten-3beta-ol-17-one + NAD+
-
Digitalis lanata 5-androsten-3,17-dione + NADH
-
r
5alpha-pregnane-3,20-dione + NADH
-
Digitalis lanata 5alpha-pregnane-3beta-ol-20-one + NAD+
-
r
5alpha-pregnane-3beta-ol-20-one + NAD+
-
Digitalis lanata 5alpha-pregnane-3,20-dione + NADH
-
r
5beta-pregnane-3,20-dione + NADH + H+
-
Digitalis lanata 5beta-pregnane-3beta-ol-20-one + 5beta-pregnane-3alpha-ol-20-one + NAD+
-
r
5beta-pregnane-3beta-ol-20-one + NAD+
-
Digitalis lanata 5beta-pregnane-3,20-dione + NADH + H+
-
r
additional information no substrate: cholesterol Digitalis lanata ?
-
?
pregnenolone + NAD+
-
Digitalis lanata isoprogesterone + progesterone + NADH + H+ about 90% odf product is isoprogesterone r

Subunits

Subunits Comment Organism
? x * 28561, calculated Digitalis lanata