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Literature summary for 1.1.1.142 extracted from

  • Ruis, H.; Hoffmann-Ostendorf, O.
    Enzymic epimerization of sequoyitol to D-pinitol in Trifolium incarnatum (1969), Eur. J. Biochem., 7, 442-448.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 21% inhibition at 0.1 mM Trifolium incarnatum
Fe2+ 25% inhibition at 0.1 mM Trifolium incarnatum
p-chloromercuribenzoate 25% inhibition at 1 mM Trifolium incarnatum
Phenylmercurinitrate 15% inhibition at 0.1 mM Trifolium incarnatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 gel filtration Trifolium incarnatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5D-5-O-methyl-chiro-inositol + NADP+ Trifolium incarnatum A-site specificity 5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Trifolium incarnatum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Trifolium incarnatum

Source Tissue

Source Tissue Comment Organism Textmining
plant prior to formation of flowers Trifolium incarnatum
-

Storage Stability

Storage Stability Organism
2°C, 0.01 M phosphate buffer, pH 7.2, little loss of activity within 3 weeks Trifolium incarnatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4,6/3,5-pentahydroxycyclohexanone + NADPH
-
 Trifolium incarnatum ? + NADP+
-
 ?
5D-5-O-methyl-chiro-inositol + NADP+ A-site specificity Trifolium incarnatum 5D-5-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH + H+
-
 r
D-2,3,5/4,6-pentahydroxycyclohexanone + NADPH
-
 Trifolium incarnatum ? + NADP+
-
 ?
dihydroxyacetone + NADPH low activity Trifolium incarnatum propanetriol + NADP+
-
 ?
DL-2,3,4,6/5-pentahydroxycyclohexanone + NADPH low activity Trifolium incarnatum ? + NADP+
-
 ?
L-2,3,5/4,6-pentahydroxycyclohexanone + NADPH low activity Trifolium incarnatum ? + NADP+
-
 ?
L-3-O-methyl-2,3,5/4,6-pentahydroxycyclohexanone + NADPH low activity Trifolium incarnatum ? + NADP+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 9
-
 Trifolium incarnatum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Trifolium incarnatum