Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.140 extracted from

  • Ladero, V.; Ramos, A.; Wiersma, A.; Goffin, P.; Schanck, A.; Kleerebezem, M.; Hugenholtz, J.; Smid, E.J.; Hols, P.
    High-level production of the low-calorie sugar sorbitol by Lactobacillus plantarum through metabolic engineering (2007), Appl. Environ. Microbiol., 73, 1864-1872.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis constitutive expression of the two sorbitol-6-phosphate dehydrogenase genes srlD1 and srlD2 in a mutant strain deficient for both L- and D-lactate dehydrogenase activities. Both Stl6PDH enzymes are active, and high specific activity can be detected in the overexpressing strains. Using resting cells under pH control with glucose as a substrate, both Stl6PDHs are capable of rerouting the glycolytic flux from fructose-6-phosphate toward sorbitol production with a remarkably high efficiency of 61 to 65% glucose conversion Lactiplantibacillus plantarum

Cloned(Commentary)

Cloned (Comment) Organism
constitutive expression in Lactobacillus plantarum Lactiplantibacillus plantarum

Protein Variants

Protein Variants Comment Organism
additional information constitutive expression of the two sorbitol-6-phosphate dehydrogenase genes srlD1 and srlD2 in a mutant strain deficient for both L- and D-lactate dehydrogenase activities. Both Stl6PDH enzymes are active, and high specific activity can be detected in the overexpressing strains. Using resting cells under pH control with glucose as a substrate, both Stl6PDHs are capable of rerouting the glycolytic flux from fructose-6-phosphate toward sorbitol production with a remarkably high efficiency of 61 to 65% glucose conversion Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-

Synonyms

Synonyms Comment Organism
Stl6PDH
-
Lactiplantibacillus plantarum