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Literature summary for 1.1.1.14 extracted from

  • Shinjoh, M.; Tomiyama, N.; Miyazaki, T.; Hoshino, T.
    Main polyol dehydrogenase of Gluconobacter suboxydans IFO 3255, membrane-bound D-sorbitol dehydrogenase, that needs product of upstream gene, sldB, for activity (2002), Biosci. Biotechnol. Biochem., 66, 2314-2322.
    View publication on PubMed
Search for more literature for 1.1.1.14

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme needs product, i.e. SldB, of upstream gene sldB, encoding a hydrophobic polypeptide, for activity, the polypeptide might be a chaperone-like component that signals processing and folding of the enzyme to give the active enzyme form Gluconobacter oxydans

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Gluconobacter oxydans

Protein Variants

Protein Variants Comment Organism
additional information construction of disruption mutants of genes sldA or sldB, the mutants are inactive, the sldB mutation leads to a higher enzyme expression and accumulation of unprocessed SldA Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Gluconobacter oxydans 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-sorbitol + NAD+ Gluconobacter oxydans enzyme is the main L-sorbose producing activity in the cell L-sorbose + NADH + H+
-
 ?
D-sorbitol + NAD+ Gluconobacter oxydans IFO 3255 enzyme is the main L-sorbose producing activity in the cell L-sorbose + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
 gene sldA
-
Gluconobacter oxydans IFO 3255
-
 gene sldA
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity at growth in media with different pH values Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol + NAD+
-
 Gluconobacter oxydans L-sorbose + NADH + H+
-
 ?
D-sorbitol + NAD+ enzyme is the main L-sorbose producing activity in the cell Gluconobacter oxydans L-sorbose + NADH + H+
-
 ?
D-sorbitol + NAD+
-
 Gluconobacter oxydans IFO 3255 L-sorbose + NADH + H+
-
 ?
D-sorbitol + NAD+ enzyme is the main L-sorbose producing activity in the cell Gluconobacter oxydans IFO 3255 L-sorbose + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
D-sorbitol dehydrogenase
-
 Gluconobacter oxydans
polyol dehydrogenase
-
 Gluconobacter oxydans
SldA
-
 Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Gluconobacter oxydans