Literature summary for 1.1.1.14 extracted from

Darmanin, C.; Iwata, T.; Carper, D.A.; Sparrow, L.G.; Chung, R.P.; El-Kabbani, O.
Expression, purification and preliminary crystallographic analysis of human sorbitol dehydrogenase (2003), Acta Crystallogr. Sect. D, 59, 558-560.

Search for more literature for 1.1.1.14

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enyme, hanging drop vapour diffusion method, 22°C, enzyme is complexed with cofactor NAD+ in a molar ratio 1:6, 0.0025 ml protein solution mixed with 0.002 ml well solution containing 0.1 M Tris, pH 8.6, 0.2 M sodium acetate, 10% PEG 3350, and with 0.0005 ml 30% v/v 2-methyl-2,4-pentanediol, 1 week, X-ray diffraction structure determination and analysis at 2.75 A resolution	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Escherichia coli by ammonium sulfate precipitation, anion-exchange and dye affinity cromatography, 23fold	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7.62	-	purified recombinant enzyme	Homo sapiens

Synonyms

Synonyms	Comment	Organism
SDH	-	Homo sapiens
sorbitol dehydrogenase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.9	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)