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Literature summary for 1.1.1.14 extracted from
- Characterization of D-sorbitol dehydrogenase involved in D-sorbitol production of a methanol yeast, Candida boidinii (Kloeckera sp.) No. 2201 (1988), Agric. Biol. Chem., 52, 419-426.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Semicarbazide | 1 mM | [Candida] boidinii |  |
| Sodium azide | 10 mM stimulates 2fold | [Candida] boidinii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba(CH3COO)2 | 1 mM reduces activity to about 20% | [Candida] boidinii | |
| CoCl2 | - |
[Candida] boidinii | |
| dithiothreitol | - |
[Candida] boidinii | |
| EDTA | - |
[Candida] boidinii | |
| Na2SO4 | - |
[Candida] boidinii | |
| NiCl2 | 1 mM reduces activity to about 20% | [Candida] boidinii | |
| o-phenanthroline | - |
[Candida] boidinii | |
| Pb(CH3COO)2 | - |
[Candida] boidinii | |
| Semicarbazide | 10 mM | [Candida] boidinii | |
| ZnSO4 | - |
[Candida] boidinii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | 10 mM FeCl3 stimulates activity 2 fold | [Candida] boidinii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Candida] boidinii | - |
(Kloeckera sp.) No. 2201 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.94 | - |
partially purified | [Candida] boidinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabinose + NADH | low activity | [Candida] boidinii | ? | - |
? | |
| D-arabitol + NAD+ | D-arabinitol is identical, low activity | [Candida] boidinii | ? | - |
? | |
| D-fructose + NADH + H+ | - |
[Candida] boidinii | D-sorbitol + NAD+ | - |
? | |
| D-glucose + NADH | low activity | [Candida] boidinii | ? | - |
? | |
| D-mannitol + NAD+ | - |
[Candida] boidinii | ? | - |
? | |
| D-ribitol + NAD+ | - |
[Candida] boidinii | ? | - |
? | |
| D-ribose + NADH | low activity | [Candida] boidinii | ? | - |
? | |
| D-ribulose + NADH | - |
[Candida] boidinii | ? | - |
? | |
| D-sorbitol + NAD+ | - |
[Candida] boidinii | D-fructose + NADH + H+ | - |
? | |
| D-xylitol + NAD+ | - |
[Candida] boidinii | ? | - |
? | |
| D-xylose + NADH | low activity | [Candida] boidinii | ? | - |
? | |
| D-xylulose + NADH | - |
[Candida] boidinii | ? | - |
? | |
| L-iditol + NAD+ | low activity | [Candida] boidinii | ? | - |
? | |
| L-rhamnose + NADH | low activity | [Candida] boidinii | ? | - |
? | |
| L-sorbose + NADH | low activity | [Candida] boidinii | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
[Candida] boidinii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
D-fructose reduction | [Candida] boidinii |
| 9.5 | - |
sorbitol oxidation | [Candida] boidinii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | 12 h at 4°C | [Candida] boidinii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | preferred | [Candida] boidinii | |
| NADH | preferred | [Candida] boidinii | |
| NADP+ | - |
[Candida] boidinii | |
| NADPH | - |
[Candida] boidinii | |
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