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Literature summary for 1.1.1.14 extracted from

  • Rizzi, M.; Harwart, K.; Erlemann, P.; Bui-Thanh, N.A.; Dellweg, H.
    Purification and properties of the NAD+-xylitol-dehydrogenase from the yeast Pichia stipitis (1989), J. Ferment. Bioeng., 67, 20-24.
No PubMed abstract available
Search for more literature for 1.1.1.14

Inhibitors

Inhibitors Comment Organism Structure
ADP 2 mM, 72% activity Scheffersomyces stipitis
AMP 2 mM, 83% activity Scheffersomyces stipitis
ATP 2 mM, 54% activity Scheffersomyces stipitis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 NAD+
-
 Scheffersomyces stipitis
26
-
 D-xylitol
-
 Scheffersomyces stipitis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE Scheffersomyces stipitis
63000
-
 gel filtration Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Scheffersomyces stipitis
-
 D-xylulose + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Scheffersomyces stipitis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58.1
-
-
 Scheffersomyces stipitis

Storage Stability

Storage Stability Organism
-60°C, 5 mM 2-mercaptoethanol, several months Scheffersomyces stipitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
 Scheffersomyces stipitis D-sorbitol + NAD+
-
 ?
D-ribitol + NAD+
-
 Scheffersomyces stipitis ?
-
 ?
D-sorbitol + NAD+
-
 Scheffersomyces stipitis D-fructose + NADH + H+
-
 ?
D-xylitol + NAD+
-
 Scheffersomyces stipitis ?
-
 ?
D-xylulose + NADH
-
 Scheffersomyces stipitis ?
-
 ?
erythritol + NAD+
-
 Scheffersomyces stipitis ?
-
 ?
L-iditol + NAD+
-
 Scheffersomyces stipitis ?
-
 ?
L-sorbose + NADH
-
 Scheffersomyces stipitis ?
-
 ?
xylitol + NAD+
-
 Scheffersomyces stipitis D-xylulose + NADH + H+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Scheffersomyces stipitis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Scheffersomyces stipitis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50 50% of maximal activity at 15°C, 40% of maximal activity at 50°C Scheffersomyces stipitis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 sorbitol oxidation Scheffersomyces stipitis

pH Range

pH Minimum pH Maximum Comment Organism
7 10 40% of maximal activity at 7 and 70% of maximal activity at 10, sorbitol oxidation Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for Scheffersomyces stipitis
NADH specific for Scheffersomyces stipitis