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Literature summary for 1.1.1.14 extracted from

  • Leissing, N.; Mc Guinness, E.T.
    Rapid affinity purification and properties of rat liver sorbitol dehydrogenase (1978), Biochim. Biophys. Acta, 524, 254-261.
    View publication on PubMed
Search for more literature for 1.1.1.14

General Stability

General Stability Organism
0.1 mg/ml bovine serum albumin stabilizes at -20°C Rattus norvegicus
0.1 mg/ml bovine serum albumin stabilizes at room temperature Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 D-sorbitol
-
 Rattus norvegicus
110
-
 D-fructose
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 gel filtration Rattus norvegicus
97000
-
 disc gel electrophoresis Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.47
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 70% loss of activity after 1 week, 0.1 mg/ml bovine serum albumin stabilizes Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
 Rattus norvegicus D-sorbitol + NAD+
-
 ?
D-mannitol + NAD+
-
 Rattus norvegicus ?
-
 ?
D-ribitol + NAD+
-
 Rattus norvegicus ?
-
 ?
D-sorbitol + NAD+
-
 Rattus norvegicus D-fructose + NADH + H+
-
 ?
D-xylitol + NAD+
-
 Rattus norvegicus ?
-
 ?
L-iditol + NAD+
-
 Rattus norvegicus ?
-
 ?
L-sorbose + NADH
-
 Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 5 h, stable Rattus norvegicus
60
-
 10 min, 65% loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9
-
 D-fructose reduction Rattus norvegicus
8.1 8.5 sorbitol oxidation Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 7 at pH 5.0 and pH 7.0 about 60% of maximal activity, fructose reduction Rattus norvegicus
7.5 9.5 at pH 7.5 and pH 9.5 about 70% of maximal activity, sorbitol oxidation Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+ specific for Rattus norvegicus
NADH specific for Rattus norvegicus
NADP+ cannot replace NAD+ Rattus norvegicus