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Literature summary for 1.1.1.138 extracted from
- Purification and characterization of mannitol dehydrogenase and identification of the corresponding cDNA from the head blight fungus, Gibberella zeae (Fusarium graminearum) (2002), Phytochemistry, 61, 791-796.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose | inhibitory above 600 mM | Fusarium graminearum |  |
| NADPH | inhibitory above 0.3 mM | Fusarium graminearum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
NADPH | cosubstrate fructose, pH 7.0, 30°C | Fusarium graminearum | |
| 0.078 | - |
NADP+ | cosubstrate mannitol, pH 9.0, 30°C | Fusarium graminearum | |
| 0.14 | - |
NADP+ | cosubstrate mannitol, pH 7.0, 30°C | Fusarium graminearum | |
| 40 | - |
D-mannitol | cosubstrate NADP+, pH 9.0, 30°C | Fusarium graminearum | |
| 57 | - |
D-mannitol | cosubstrate NADP+, pH 7.0, 30°C | Fusarium graminearum | |
| 290 | - |
D-fructose | cosubstrate NADPH, pH 7.0, 30°C | Fusarium graminearum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
4 * 29000, SDS-PAGE | Fusarium graminearum |
| 110000 | - |
gel filtration | Fusarium graminearum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium graminearum | - |
Fusarium graminearum | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fusarium graminearum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.54 | - |
pH 9.0, 30°C | Fusarium graminearum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADPH | - |
Fusarium graminearum | D-mannitol + NADP+ | - |
r | |
| D-mannitol + NADP+ | - |
Fusarium graminearum | D-fructose + NADPH + H+ | - |
r | |
| L-sorbitol + NADP+ | 21% of the activity with D-mannitol | Fusarium graminearum | L-sorbose + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 29000, SDS-PAGE | Fusarium graminearum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
D-fructose reduction | Fusarium graminearum |
| 9 | - |
D-mannitol oxidation | Fusarium graminearum |
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