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Literature summary for 1.1.1.138 extracted from
- Purification and characterization of a novel mannitol dehydrogenase from Lactobacillus intermedius (2004), Biotechnol. Prog., 20, 537-542.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM, 60% inhibition | Lactobacillus intermedius |  |
| additional information | not inhibitory: CaCl2, MgCl2, Cocl2, dithiothreitol | Lactobacillus intermedius | |
| p-chloromercuribenzoate | 0.2 mM, complete inhibition | Lactobacillus intermedius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20 | - |
D-fructose | pH 5.5, 30°C | Lactobacillus intermedius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34500 | - |
2 * 43000 + 2 * 34500, SDS-PAGE, both subunits have the same amino-terminal amino acid sequence | Lactobacillus intermedius |
| 43000 | - |
2 * 43000 + 2 * 34500, SDS-PAGE, both subunits have the same amino-terminal amino acid sequence | Lactobacillus intermedius |
| 170000 | - |
gel filtration | Lactobacillus intermedius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus intermedius | - |
NRRL B-3693, enzyme production depends on growth on fructose or sucrose | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactobacillus intermedius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 331 | - |
pH 5.5, 30°C | Lactobacillus intermedius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADPH | - |
Lactobacillus intermedius | D-mannitol + NADP+ | - |
r | |
| D-mannitol + NAD+ | less than 25% of the activity with NADP+ | Lactobacillus intermedius | D-fructose + NADH + H+ | - |
r | |
| D-mannitol + NADP+ | - |
Lactobacillus intermedius | D-fructose + NADPH + H+ | - |
r | |
| additional information | no substrate: glucose, xylose, arabinose | Lactobacillus intermedius | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | 2 * 43000 + 2 * 34500, SDS-PAGE, both subunits have the same amino-terminal amino acid sequence | Lactobacillus intermedius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
reduction of D-fructose | Lactobacillus intermedius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
reduction of D-fructose, 44% of maximum activity | Lactobacillus intermedius |
| 60 | - |
reduction of D-fructose, 15% of maximum activity | Lactobacillus intermedius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
stable up to | Lactobacillus intermedius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
reduction of D-fructose | Lactobacillus intermedius |
| 7 | - |
oxidation of D-fructose | Lactobacillus intermedius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
reduction of D-fructose, 35% of maximum activity | Lactobacillus intermedius |
| 6.5 | - |
reduction of D-fructose, 50% of maximum activity | Lactobacillus intermedius |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | - |
Lactobacillus intermedius |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Lactobacillus intermedius | |
| NADPH | - |
Lactobacillus intermedius | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Lactobacillus intermedius | isoelectric focusing | - |
4.9 |
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