Literature summary for 1.1.1.138 extracted from

Lee, J.K.; Koo, B.S.; Kim, S.Y.; Hyun, H.H.
Purification and characterization of a novel mannitol dehydrogenase from a newly isolated strain of Candida magnoliae (2003), Appl. Environ. Microbiol., 69, 4438-4447.

Search for more literature for 1.1.1.138

Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethanol	0.5 mM, complete inhibition	Starmerella magnoliae
Cu2+	-	Starmerella magnoliae
D-mannitol	-	Starmerella magnoliae
dithiothreitol	0.5 mM, complete inhibition	Starmerella magnoliae
glutathione	0.5 mM, complete inhibition	Starmerella magnoliae
L-cysteine	0.5 mM, complete inhibition	Starmerella magnoliae
NADP+	-	Starmerella magnoliae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0578	-	NADPH	pH 7.5, 37°C	Starmerella magnoliae
0.612	-	NADH	pH 7.5, 37°C	Starmerella magnoliae
28	-	D-fructose	pH 7.5, 37°C	Starmerella magnoliae
212	-	ribose	pH 7.5, 37°C	Starmerella magnoliae
352	-	D-mannose	pH 7.5, 37°C	Starmerella magnoliae
385	-	arabinose	pH 7.5, 37°C	Starmerella magnoliae
402	-	xylose	pH 7.5, 37°C	Starmerella magnoliae
680	-	D-mannitol	pH 7.5, 37°C	Starmerella magnoliae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no stimulation by: MgCl2, MnCl2, ZnCl2, NiCl2, CoCl2, FeCl2	Starmerella magnoliae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	4 * 35000, SDS-PAGE	Starmerella magnoliae
142000	-	gel filtration	Starmerella magnoliae

Organism

Organism	UniProt	Comment	Textmining
Starmerella magnoliae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Starmerella magnoliae

Reaction

Reaction	Comment	Organism	Reaction ID
D-mannitol + NADP+ = D-fructose + NADPH + H+	sequential ordered bi-bi mechanism	Starmerella magnoliae	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1	-	substrate D-fructose + NADH, pH 7.5, 37°C	Starmerella magnoliae
3.2	-	substrate arabinose, pH 7.5, 37°C	Starmerella magnoliae
5.1	-	substrate xylose, pH 7.5, 37°C	Starmerella magnoliae
5.2	-	substrate ribose, pH 7.5, 37°C	Starmerella magnoliae
6.1	-	substrate D-mannose, pH 7.5, 37°C	Starmerella magnoliae
9.1	-	substrate D-mannitol, pH 7.5, 37°C	Starmerella magnoliae
315	-	substrate D-fructose + NADPH, pH 7.5, 37°C	Starmerella magnoliae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arabinose + NADPH + H+	-	Starmerella magnoliae	arabinitol + NADP+	-	r
D-fructose + NADPH	-	Starmerella magnoliae	D-mannitol + NADP+	-	r
D-mannitol + NAD+	-	Starmerella magnoliae	D-fructose + NADH + H+	-	r
D-mannitol + NADP+	less than 3% the rate of fructose reduction	Starmerella magnoliae	D-fructose + NADPH + H+	-	r
D-mannose + NADPH + H+	-	Starmerella magnoliae	D-mannitol + NADP+	-	r
additional information	specific for transfer of 4-pro-S hydrogen of NADPH	Starmerella magnoliae	?	-	?
ribose + NADPH + H+	-	Starmerella magnoliae	ribitol + NADP+	-	r
xylose + NADPH + H+	-	Starmerella magnoliae	xylitol + NADP+	-	r

Subunits

Subunits	Comment	Organism
tetramer	4 * 35000, SDS-PAGE	Starmerella magnoliae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	reduction of fructose	Starmerella magnoliae
40	-	oxidation ofr fructose	Starmerella magnoliae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	reduction of fructose	Starmerella magnoliae
10	-	oxidation of fructose	Starmerella magnoliae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0011	-	Cu2+	pH 7.5, 37°C	Starmerella magnoliae
0.18	-	NADP+	cosubstrate fructose, pH 7.5, 37°C	Starmerella magnoliae
0.21	-	NADP+	cosubstrate NADPH, pH 7.5, 37°C	Starmerella magnoliae
188	-	mannitol	pH 7.5, 37°C	Starmerella magnoliae

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Release 2023.1 (January 2023)