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Literature summary for 1.1.1.133 extracted from

  • Parakkottil Chothi, M.; Duncan, G.; Armirotti, A.; Abergel, C.; Gurnon, J.; Van Etten, J.; Bernardi, C.; Damonte, G.; Tonetti, M.
    Identification of an L-rhamnose synthetic pathway in two nucleocytoplasmic large DNA viruses (2010), J. Virol., 84, 8829-8838.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene L780, transcription profiling of UGER, phylogenetic analysis Acanthamoeba polyphaga Mimivirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.83
-
UDP-4-keto-6-deoxy-D-glucose 25°C, pH 7.5 Acanthamoeba polyphaga Mimivirus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information divalent metal ions do not alter activity Acanthamoeba polyphaga Mimivirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, UGER, SDS-PAGE Acanthamoeba polyphaga Mimivirus
47000
-
gel filtration Acanthamoeba polyphaga Mimivirus

Organism

Organism UniProt Comment Textmining
Acanthamoeba polyphaga Mimivirus E3VXL5 gene L780
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0011
-
25°C, pH 7.5 Acanthamoeba polyphaga Mimivirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-4-keto-6-deoxy-D-glucose + NADPH + H+
-
Acanthamoeba polyphaga Mimivirus UDP-L-rhamnose + NADP+ product identification by electrospray ionization-mass spectrometry and gas chromatography mass spectrometry ?

Subunits

Subunits Comment Organism
monomer or dimer x * 33000, UGER, SDS-PAGE Acanthamoeba polyphaga Mimivirus

Synonyms

Synonyms Comment Organism
bifunctional UDP-4-keto-6-deoxy-D-glucose epimerase/reductase
-
Acanthamoeba polyphaga Mimivirus
UGER
-
Acanthamoeba polyphaga Mimivirus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
stable for 30 min Acanthamoeba polyphaga Mimivirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Acanthamoeba polyphaga Mimivirus

Cofactor

Cofactor Comment Organism Structure
NADPH specific Acanthamoeba polyphaga Mimivirus