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Literature summary for 1.1.1.133 extracted from

  • Graninger, M.; Nidetzky, B.; Heinrichs, D.E.; Whitfield, C.; Messner, P.
    Characterization of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase, required for dTDP-L-rhamnose biosynthesis in Salmonella enterica serovar typhimurium LT2 (1999), J. Biol. Chem., 274, 25069-25077.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli BL21(lambdaDE3) Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
EDTA up to 0.3 mM, 70% loss of enzyme activity Salmonella enterica
additional information full activity restored with up to 0.3 mM MgCl2 Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.106
-
 NADH +/-0.018, pH 7.0 Salmonella enterica
0.21
-
 NADH +/-0.004, pH 7.0 Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32600
-
 calculated from nucleotide sequence data Salmonella enterica
34800
-
 SDS-PAGE Salmonella enterica
41500
-
 gel filtration Salmonella enterica

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
 serovar typhimurium LT2
-

Purification (Commentary)

Purification (Comment) Organism
affinity and ion exchange chromatography Salmonella enterica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44
-
-
 Salmonella enterica

Storage Stability

Storage Stability Organism
-20°C, 44% glycerol Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+
-
 Salmonella enterica dTDP-beta-L-rhamnose + NAD+
-
 ?
dTDP-6-deoxy-L-lyxo-4-hexulose + NAD(P)H
-
 Salmonella enterica dTDP-L-rhamnose + NAD(P)+
-
 r

Synonyms

Synonyms Comment Organism
dTDP-4-dehydrorhamnose reductase
-
 Salmonella enterica
thymidine diphosphate-4-dehydrorhamnose reductase
-
 Salmonella enterica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable below Salmonella enterica
37
-
 pH 7.0, 75% activity loss Salmonella enterica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25
-
 NADH +/-48 Salmonella enterica
29
-
 NADPH +/-66 Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 reduction of dTDP-6-deoxy-L-lyxo-4-hexulose Salmonella enterica
10
-
 oxidation of dTDP-L-rhamnose Salmonella enterica

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 37°C, 20 min, 75% activity loss Salmonella enterica

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Salmonella enterica
NADH preferred Salmonella enterica
NADP+
-
 Salmonella enterica
NADPH
-
 Salmonella enterica