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Literature summary for 1.1.1.133 extracted from

  • Giraud, M.F.; McMiken, H.J.; Leonard, G.A.; Messner, P.; Whitfield, C.; Naismith, J.H.
    Overexpression, purification, crystallization and preliminary structural study of dTDP-6-deoxy-L-lyxo-4-hexulose reductase (RmlD), the fourth enzyme of the dTDP-L-rhamnose synthesis pathway, from Salmonella enterica serovar typhimurium (1999), Acta Crystallogr. Sect. D, 55, 2043-2046.
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Cloned(Commentary)

Cloned (Comment) Organism
native overexpressed in Escherichia coli BL21(DE3) and selenomethionine-enriched in B834(lambdaDE3) Salmonella enterica

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour-diffusion method, 6 d Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
 HPLC Salmonella enterica
32540
-
 mass spectrometry, native protein Salmonella enterica
32560
-
 deduced from sequence Salmonella enterica
32730
-
 mass spectrometry, selenomethionine-enriched protein Salmonella enterica
49500
-
 light-scattering Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH Escherichia coli synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria dTDP-L-rhamnose + NADP+
-
 ?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH Xanthomonas campestris synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria dTDP-L-rhamnose + NADP+
-
 ?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH Salmonella enterica synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria dTDP-L-rhamnose + NADP+
-
 ?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH Shigella flexneri synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria dTDP-L-rhamnose + NADP+
-
 ?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH Streptomyces mutans synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria dTDP-L-rhamnose + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K12
-
Salmonella enterica
-
-
-
Shigella flexneri
-
-
-
Streptomyces mutans
-
-
-
Xanthomonas campestris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange, hydrophobic chromatography Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria Escherichia coli dTDP-L-rhamnose + NADP+
-
 ?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria Xanthomonas campestris dTDP-L-rhamnose + NADP+
-
 ?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria Salmonella enterica dTDP-L-rhamnose + NADP+
-
 ?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria Shigella flexneri dTDP-L-rhamnose + NADP+
-
 ?
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria Streptomyces mutans dTDP-L-rhamnose + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
-
 Salmonella enterica

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Salmonella enterica
NADPH
-
 Shigella flexneri
NADPH
-
 Xanthomonas campestris
NADPH
-
 Escherichia coli
NADPH
-
 Streptomyces mutans