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Literature summary for 1.1.1.130 extracted from
- beta-Keto-L-gulonic acid as an intermediate in the bacterial metabolism of ascorbic acid (1962), J. Biol. Chem., 237, 2454-2457.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | 32% inhibition at 1 mM, not reversal by GSH | Klebsiella aerogenes |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
NADH | - |
Klebsiella aerogenes | |
| 0.022 | - |
NADPH | - |
Klebsiella aerogenes | |
| 1.16 | - |
(4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate | - |
Klebsiella aerogenes | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H | Klebsiella aerogenes | - |
3-dehydro-L-gulonate + NAD(P)+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Klebsiella aerogenes |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Klebsiella aerogenes | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.06 | - |
- |
Klebsiella aerogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 0.03 M Tris-HCl, pH 7.7, 0.62 mg protein/ml, 85% retaining activity in 4 weeks | Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H | - |
Klebsiella aerogenes | 3-dehydro-L-gulonate + NAD(P)+ | - |
? | |
| 2,3-diketogulonic acid + NADH + H+ | - |
Klebsiella aerogenes | 3-dehydro-L-gulonate + NAD+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Klebsiella aerogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 8.4 | 50% of maximal activity at pH 5.2 and pH 8.4 | Klebsiella aerogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | twice effective compared to NADPH | Klebsiella aerogenes | |
| NADPH | NADH 2fold more effective | Klebsiella aerogenes | |
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Release 2023.1 (January 2023)
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