Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.122 extracted from

  • Brouns, S.J.; Turnbull, A.P.; Willemen, H.L.; Akerboom, J.; van der Oost, J.
    Crystal structure and biochemical properties of the D-arabinose dehydrogenase from Sulfolobus solfataricus (2007), J. Mol. Biol., 371, 1249-1260.
    View publication on PubMed
Search for more literature for 1.1.1.122

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure is solved to a resolution of 1.80 A by single-wavelength anomalous diffraction and phased using the two endogenous zinc ions per subunit. The structure reveals a catalytic and cofactor binding domain Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains two endogenous zinc ions per subunit.A zinc ion is bound in the loop that protrudes from the catalytic domain. This zinc is tetrahedrally coordinated by residues D94, C97, C100 and C108 Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97YM2
-
-
Saccharolobus solfataricus P2 Q97YM2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-deoxy-L-galactose + NAD+ i.e. L-fucose. The enzyme is specific for sugars with D-arabinose-type hydroxyl group stereo-configurations at C3 and C4. This configuration is shared among the hexoses L-fucose (6-deoxy-L-galactose) and L-galactose, and the pentose D-ribose. Although the highest activity is observed with L-fucose in combination with NAD+, the remaining sugars are oxidized at higher rates using NADP+ Saccharolobus solfataricus 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
6-deoxy-L-galactose + NAD+ i.e. L-fucose. The enzyme is specific for sugars with D-arabinose-type hydroxyl group stereo-configurations at C3 and C4. This configuration is shared among the hexoses L-fucose (6-deoxy-L-galactose) and L-galactose, and the pentose D-ribose. Although the highest activity is observed with L-fucose in combination with NAD+, the remaining sugars are oxidized at higher rates using NADP+ Saccharolobus solfataricus P2 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme is specific for sugars with D-arabinose-type hydroxyl group stereo-configurations at C3 and C4. This configuration is shared among the hexoses L-fucose (6-deoxy-L-galactose) and L-galactose, and the pentose D-ribose. Although the highest activity is observed with L-fucose in combination with NAD+, the remaining sugars are oxidized at higher rates using NADP+ Saccharolobus solfataricus