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Literature summary for 1.1.1.122 extracted from

  • Schacter, H.; Sarney, J.; McGuire, E.J.; Roseman, S.
    Isolation of diphosphopyridine nucleotide-dependent L-fucose dehydrogenase from pork liver (1969), J. Biol. Chem., 244, 4785-4792.
    View publication on PubMed
Search for more literature for 1.1.1.122

Activating Compound

Activating Compound Comment Organism Structure
hydroxylamine stimulation at high concentrations Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
3-Amino-3-deoxy-D-arabinose
-
 Sus scrofa
D-arabinose competitive inhibition of L-fucose oxidation at 2.5 mM Sus scrofa
p-hydroxymercuribenzoate 20% loss of activity, saturated Sus scrofa
sodium iodoacetate 20% loss of activity at 20 mM, pH 8.0 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NAD+
-
 Sus scrofa
0.32
-
 6-deoxy-L-galactose
-
 Sus scrofa
2.1
-
 D-arabinose
-
 Sus scrofa
8
-
 L-galactose
-
 Sus scrofa
8
-
 3-Amino-3-deoxy-D-arabinose
-
 Sus scrofa
44
-
 L-Xylose
-
 Sus scrofa
48
-
 D-Lyxose
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-amino-3-deoxy-D-arabinose + NAD+ Sus scrofa 15% of the activity compared to L-fucose 3-amino-3-deoxy-D-arabino-1,5-lactone + NADH
-
 ?
6-deoxy-L-galactose + NAD+ Sus scrofa i.e. L-fucose 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
D-arabinose + NAD+ Sus scrofa 66% of the activity compared to L-fucose D-arabinono-1,4-lactone + NADH
-
 ?
D-lyxose + NAD+ Sus scrofa 4% of the activity compared to L-fucose ? + NADH
-
 ?
L-galactose + NAD+ Sus scrofa 13% of the activity compared to L-fucose L-galactono-1,5-lactone + NADH
-
 ?
L-xylose + NAD+ Sus scrofa 4% of the activity compared to L-fucose L-xylono-1,5-lactone + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 porcine
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps, preparative gel electrophoresis Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
433
-
 purified enzyme Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-amino-3-deoxy-D-arabinose + NAD+ 15% of the activity compared to L-fucose Sus scrofa 3-amino-3-deoxy-D-arabino-1,5-lactone + NADH
-
 ?
3-amino-3-deoxy-D-arabinose + NAD+
-
 Sus scrofa 3-amino-3-deoxy-D-arabino-1,5-lactone + NADH + H+
-
 r
6-deoxy-L-galactose + NAD+ i.e. L-fucose Sus scrofa 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
D-arabinose + NAD+ 66% of the activity compared to L-fucose Sus scrofa D-arabinono-1,4-lactone + NADH
-
 ?
D-lyxose + NAD+ 4% of the activity compared to L-fucose Sus scrofa ? + NADH
-
 ?
L-galactose + NAD+ 13% of the activity compared to L-fucose Sus scrofa L-galactono-1,5-lactone + NADH
-
 ?
L-xylose + NAD+ 4% of the activity compared to L-fucose Sus scrofa L-xylono-1,5-lactone + NADH
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
 Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD-specific Sus scrofa