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Literature summary for 1.1.1.122 extracted from
- The physical properties of NAD-dependent L-fucose dehydrogenase from sheep liver (1978), Arch. Biochem. Biophys., 186, 184-188.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KNO3 | activation at 1-1.3 M, optimal pH shifts to 9-9.5 | Ovis aries aries |  |
| NaBr | activation at 1-1.3 M, optimal pH shifts to 9-9.5 | Ovis aries aries | |
| NaCl | activation at 1-1.3 M, optimal pH shifts to 9-9.5 | Ovis aries aries | |
| NaNO3 | activation at 1-1.3 M, optimal pH shifts to 9-9.5 | Ovis aries aries | |
| Tris-HCl | activation at 1-1.3 M, optimal pH shifts to 9-9.5 | Ovis aries aries | |
General Stability
| General Stability | Organism |
|---|---|
| NAD+ stabilizes | Ovis aries aries |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | slight inhibition | Ovis aries aries | |
| ATP | slight inhibition | Ovis aries aries | |
| NADH | Ki: 0.04 mM | Ovis aries aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
L-galactose | - |
Ovis aries aries | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration | Ovis aries aries |
| 35000 | - |
x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration | Ovis aries aries |
| 82000 | 123000 | sedimentation equilibrium analysis: 123000 Da, gel filtration: 82000 Da | Ovis aries aries |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-deoxy-L-galactose + NAD+ | Ovis aries aries | i.e. L-fucose | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
? | |
| L-galactose + NAD+ | Ovis aries aries | - |
L-galactono-1,5-lactone + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries aries | - |
sheep | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques | Ovis aries aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Ovis aries aries | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 7.2, half-life: 39 days | Ovis aries aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-deoxy-L-galactose + NAD+ | i.e. L-fucose | Ovis aries aries | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
? | |
| L-galactose + NAD+ | - |
Ovis aries aries | L-galactono-1,5-lactone + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE, x * 35000, guanidinium-HCl gel filtration | Ovis aries aries |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
at pH 7.2: half-life: 1.8 days | Ovis aries aries |
| 60 | - |
at pH 7.2, half-life: 25 min without added NAD+, half-life: 35 min including added NAD+, at pH 8.5: activity more rapidly lost | Ovis aries aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Ovis aries aries | |
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Release 2023.1 (January 2023)
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