Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.122 extracted from

  • Tsuji, Y.; Koike, A.; Yamamoto, K.; Tochikura, T.
    Purification and some properties of L-fucose dehydrogenase from Agrobacterium radiobacter and its application to the assay of bound-fucose in glycoconjugates (1992), Biochim. Biophys. Acta, 1117, 167-173.
    View publication on PubMed
Search for more literature for 1.1.1.122

Application

Application Comment Organism
analysis determination of bound-fucose in biological materials by a coupled enzymatic method in a single buffer system Agrobacterium tumefaciens

General Stability

General Stability Organism
sensitive to repeated freezing and thawing Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 1 mM Agrobacterium tumefaciens
Hg2+ complete inhibition at 1 mM Agrobacterium tumefaciens
N-ethylmaleimide strong inhibition at 0.1 mM Agrobacterium tumefaciens
p-chloromercuribenzoate strong inhibition at 0.1 mM Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NADP+
-
 Agrobacterium tumefaciens
0.61
-
 NAD+
-
 Agrobacterium tumefaciens
3
-
 6-deoxy-L-galactose
-
 Agrobacterium tumefaciens
14.3
-
 D-arabinose
-
 Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 1 * 37000 + 1 * 28000, SDS-PAGE Agrobacterium tumefaciens
37000
-
 1 * 37000 + 1 * 28000, SDS-PAGE Agrobacterium tumefaciens
64000
-
 gel filtration Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-deoxy-L-galactose + NAD+ Agrobacterium tumefaciens i.e. L-fucose 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
D-arabinose + NAD+ Agrobacterium tumefaciens 71% of the activity compared to L-fucose D-arabinono-1,4-lactone + NADH
-
 ?
L-galactose + NAD+ Agrobacterium tumefaciens 57% of the activity compared to L-fucose L-galactono-1,5-lactone + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Agrobacterium tumefaciens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Agrobacterium tumefaciens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53
-
 purified enzyme Agrobacterium tumefaciens

Storage Stability

Storage Stability Organism
-30°C, 6 months Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-deoxy-L-galactose + NAD+ i.e. L-fucose Agrobacterium tumefaciens 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
D-arabinose + NAD+ 71% of the activity compared to L-fucose Agrobacterium tumefaciens D-arabinono-1,4-lactone + NADH
-
 ?
fuconolactone + NADPH + H+
-
 Agrobacterium tumefaciens L-fucose + NADP+
-
 ?
L-galactose + NAD+ 57% of the activity compared to L-fucose Agrobacterium tumefaciens L-galactono-1,5-lactone + NADH
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 37000 + 1 * 28000, SDS-PAGE Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable up to Agrobacterium tumefaciens

pH Range

pH Minimum pH Maximum Comment Organism
7 9 active within Agrobacterium tumefaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9.5 stable within Agrobacterium tumefaciens

Cofactor

Cofactor Comment Organism Structure
NAD+ also able to use NADP+ Agrobacterium tumefaciens
NADP+ 30% of the activity compared to NAD+, L-fucose dehydrogenation Agrobacterium tumefaciens