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Literature summary for 1.1.1.121 extracted from

  • Dahms, A.S.; Anderson, R.L.
    D-Fucose metabolism in a pseudomonad. I. Oxidation of D-fucose to D-fucono-delta-lactone by a D-aldohexose dehydrogenase (1972), J. Biol. Chem., 247, 2222-2227.
    View publication on PubMed
Search for more literature for 1.1.1.121

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Pseudomonas sp.
Co2+
-
 Pseudomonas sp.
Cu2+
-
 Pseudomonas sp.
Fe2+
-
 Pseudomonas sp.
additional information no inhibition by 6.7 mM EDTA or 4.0 mM NADP+ Pseudomonas sp.
NH4+
-
 Pseudomonas sp.
Ni2+
-
 Pseudomonas sp.
Zn2+
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 NAD+
-
 Pseudomonas sp.
0.86
-
 D-glucose
-
 Pseudomonas sp.
1.6
-
 D-galactose
-
 Pseudomonas sp.
1.6
-
 2-deoxy-D-glucose
-
 Pseudomonas sp.
2.4
-
 D-altrose
-
 Pseudomonas sp.
4.5
-
 D-mannose
-
 Pseudomonas sp.
5.8
-
 D-fucose
-
 Pseudomonas sp.
6.3
-
 2-deoxy-D-galactose
-
 Pseudomonas sp.
13
-
 D-allose
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-aldose + NAD+ Pseudomonas sp. broad substrate specificity D-aldonolactone + NADH
-
 ?
D-aldose + NAD+ Pseudomonas sp. D-aldoses of pyranose ring size D-aldonolactone + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 MSU-1
-
Pseudomonas sp. MSU-1
-
 MSU-1
-

Purification (Commentary)

Purification (Comment) Organism
MSU-1 Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture cells grown on various sugars, D-fucose and D-glucose are effective inducers of D-aldohexose dehydrogenase, D-galactose induces only slightly, L-arabinose is no inducer Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.8
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, Sephadex G-200 fractions are stable for at least 6 months Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-galactose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
2-deoxy-D-glucose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
D-aldose + NAD+ broad substrate specificity Pseudomonas sp. D-aldonolactone + NADH
-
 ?
D-aldose + NAD+ D-aldoses of pyranose ring size Pseudomonas sp. D-aldonolactone + NADH
-
 ?
D-allose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
D-altrose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
D-fucose + NAD+
-
 Pseudomonas sp. D-fucono-delta-lactone + NADH hydrolyzes spontaneously to D-fuconate ?
D-galactose + NAD+
-
 Pseudomonas sp. D-galactono-gamma-lactone + NADH
-
 ?
D-glucose + NAD+ the most probable produced aldonolactones from this and the following substrates have not been isolated so far Pseudomonas sp. D-glucono-delta-lactone + NADH + H+
-
 r
D-glucose + NAD+ beta-anomer preferred over alpha-anomer Pseudomonas sp. D-glucono-delta-lactone + NADH + H+
-
 r
D-glucose + NAD+ the most probable produced aldonolactones from this and the following substrates have not been isolated so far Pseudomonas sp. MSU-1 D-glucono-delta-lactone + NADH + H+
-
 r
D-mannose + NAD+
-
 Pseudomonas sp. ? + NADH
-
 ?
additional information broad substrate specificity Pseudomonas sp. ?
-
 ?
additional information substrates are D-aldoses of pyranose ring size Pseudomonas sp. ?
-
 ?
additional information L-aldohexoses, ketohexoses, pentoses, trioses, polyols, di- and trisaccharides, derivatives of D-aldohexoses are no substrates Pseudomonas sp. ?
-
 ?
additional information NADP+ ineffective as cofactor at concentrations up to 20 mM Pseudomonas sp. ?
-
 ?
additional information broad substrate specificity Pseudomonas sp. MSU-1 ?
-
 ?
additional information substrates are D-aldoses of pyranose ring size Pseudomonas sp. MSU-1 ?
-
 ?
additional information L-aldohexoses, ketohexoses, pentoses, trioses, polyols, di- and trisaccharides, derivatives of D-aldohexoses are no substrates Pseudomonas sp. MSU-1 ?
-
 ?
additional information NADP+ ineffective as cofactor at concentrations up to 20 mM Pseudomonas sp. MSU-1 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 half-life: 40 s in sodium phosphate buffer, pH 7.0, with D-fucose, D-galactose, D-glucose or D-mannose as substrate Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 Tris/HCl buffer Pseudomonas sp.
9 10 glycine/NaOH buffer Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.3 9.3 66% of maximal activity at pH 7.2 and pH 9.3 in Tris/HCl buffer Pseudomonas sp.
8.5 10.4 about 80% of maximal activity at pH 8.5 and 10.4 in glycine/NaOH buffer Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas sp.
NADP+ active at concentrations above 0.02 M Pseudomonas sp.