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Literature summary for 1.1.1.118 extracted from
- Characterization of a robust glucose 1-dehydrogenase, SyGDH, and its application in NADPH regeneration for the asymmetric reduction of haloketone by a carbonyl reductase in organic solvent/buffer system (2020), Proc. Biochem., 89, 55-62 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | application in NADPH regeneration for the asymmetric reduction of haloketone by a carbonyl reductase in organic solvent/buffer system | synthetic construct |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a codon-optimized gene Sygdh encoding glucose 1-dehydrogenase (SyGDH) is synthesized based on the putative GDH gene sequence (Ta0897) in Thermoplasma acidophilum genomic DNA, and expressed in Escherichia coli BL21(DE3) | synthetic construct |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
synthetic construct |  |
| EDTA | - |
synthetic construct | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.67 | - |
NAD+ | pH 7.5, 40°C | synthetic construct | |
| 7.48 | - |
D-glucose | pH 7.5, 40°C | synthetic construct | |
| 157.9 | - |
NADP+ | pH 7.5, 40°C | synthetic construct | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
synthetic construct | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| DMSO | retains over 90% activity after incubation in organic solvent/buffer (1:1, v/v) system at 40°C and 220 rpm for 12 h | synthetic construct |
| Ethanol | retains over 90% activity after incubation in organic solvent/buffer (1:1, v/v) system at 40°C and 220 rpm for 12 h | synthetic construct |
| isopropanol | retains over 90% activity after incubation in organic solvent/buffer (1:1, v/v) system at 40°C and 220 rpm for 12 h | synthetic construct |
| Methanol | retains over 90% activity after incubation in organic solvent/buffer (1:1, v/v) system at 40°C and 220 rpm for 12 h | synthetic construct |
| N,N-dimethylformamide | half-life of 3 h | synthetic construct |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| synthetic construct | AHI17928.1 | a codon-optimized gene Sygdh encoding glucose 1-dehydrogenase (SyGDH) is synthesized based on the putative GDH gene sequence (Ta0897) in Thermoplasma acidophilum genomic DNA, and expressed in Escherichia coli BL21(DE3) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatographys | synthetic construct |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
40°C, pH 7.5, coenzyme: NAD+ | synthetic construct |
| 86.3 | - |
40°C, pH 7.5, coenzyme: NADP+ | synthetic construct |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-galactose + NAD+ | - |
synthetic construct | D-galactono-1,5-lactone + NADH | - |
? | |
| D-glucose + NAD+ | the enzyme prefers NADP+ as coenzyme to NAD+ | synthetic construct | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| D-glucose + NADP+ | - |
synthetic construct | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
| additional information | the enzyme shows no activity towards D-xylose, D-mannose, D-maltose, glucose 6-phosphate and sucrose | synthetic construct | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucose 1-dehydrogenase | - |
synthetic construct |
| SyGDH | - |
synthetic construct |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
synthetic construct |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | 10°C: about 35% of maximal activity, 20°C: about 80% of maximal activity, 60°C: about 75% of maximal activity | synthetic construct |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 64.9 | - |
D-glucose | pH 7.5, 40°C | synthetic construct | |
| 69.7 | - |
NAD+ | pH 7.5, 40°C | synthetic construct | |
| 101.9 | - |
NADP+ | pH 7.5, 40°C | synthetic construct | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
synthetic construct |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | pH 5.5: about 50% of maximal activity, pH 8.0: about 40% of maximal activity | synthetic construct |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | the enzyme prefers NADP+ as coenzyme to NAD+ | synthetic construct | |
| NADP+ | the enzyme prefers NADP+ as coenzyme to NAD+ | synthetic construct | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.64 | - |
NADP+ | pH 7.5, 40°C | synthetic construct | |
| 8.67 | - |
D-glucose | pH 7.5, 40°C | synthetic construct | |
| 104 | - |
NAD+ | pH 7.5, 40°C | synthetic construct | |
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