General Stability | Organism |
---|---|
unstable in buffers without glycerol, stabilized by adding 30-50% glycerol to the buffer | Candida maltosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Candida maltosa | |
EDTA | - |
Candida maltosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
NADH | - |
Candida maltosa | |
0.0086 | - |
NADPH | - |
Candida maltosa | |
0.021 | - |
NADP+ | - |
Candida maltosa | |
0.044 | - |
p-hydroxyphenylpyruvate | - |
Candida maltosa | |
0.067 | - |
Indolepyruvate | - |
Candida maltosa | |
0.076 | - |
p-hydroxyphenyllactate | - |
Candida maltosa | |
0.083 | - |
D-phenyllactate | - |
Candida maltosa | |
0.093 | - |
NAD+ | - |
Candida maltosa | |
0.094 | - |
indolelactate | - |
Candida maltosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | inhibitory | Candida maltosa | |
Mn2+ | required | Candida maltosa | |
Zn2+ | inhibitory | Candida maltosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
4 * 68000, SDS-PAGE, gel filtration chromatography | Candida maltosa |
250000 | - |
gel filtration | Candida maltosa |
280000 | - |
glycerol density gradient centrifugation | Candida maltosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(4-hydroxyphenyl)pyruvate + NAD(P)H | Candida maltosa | involved in aromatic amino acid catabolism | (R)-3-(4-hydroxyphenyl)lactate + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida maltosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida maltosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
crude extract | Candida maltosa |
1170 | - |
purified enzyme | Candida maltosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ketoisopentanoate + NAD(P)H | poor substrate | Candida maltosa | 2-hydroxyisopentanoate + NAD(P)+ | - |
r | |
2-oxobutyrate + NAD(P)H + H+ | poor substrate | Candida maltosa | 2-hydroxybutyrate + NAD(P)+ | - |
r | |
3-(4-hydroxyphenyl)pyruvate + NAD(P)H | the initial rate of oxidation is 100 fold lower than that of reduction | Candida maltosa | (R)-3-(4-hydroxyphenyl)lactate + NAD(P)+ | - |
r | |
3-(4-hydroxyphenyl)pyruvate + NAD(P)H | involved in aromatic amino acid catabolism | Candida maltosa | (R)-3-(4-hydroxyphenyl)lactate + NAD(P)+ | - |
? | |
indolepyruvate + NAD(P)H | reduction at 30% the rate of p-hydroxyphenylpyruvate reduction | Candida maltosa | indolelactate + NAD(P)+ | - |
r | |
additional information | best substrate for oxidation is p-hydroxyphenyllactate, D-phenyllactate is oxidized at 75% and indolelactate at 52% the rate of p-hydroxyphenyllactate | Candida maltosa | ? | - |
? | |
phenylpyruvate + NAD(P)H | reduction at 27% the rate of p-hydroxyphenylpyruvate reduction | Candida maltosa | D-phenyllactate + NAD(P)+ | - |
r | |
pyruvate + NAD(P)H + H+ | poor substrate | Candida maltosa | lactate + NAD(P)+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 68000, SDS-PAGE, gel filtration chromatography | Candida maltosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Candida maltosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | - |
Candida maltosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
significant loss of activity above 40°C | Candida maltosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction | Candida maltosa |
9.5 | - |
oxidation | Candida maltosa |