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Literature summary for 1.1.1.107 extracted from

  • Chu, H.N.; Kobayashi, J.; Yoshikane, Y.; Mikami, B.; Yagi, T.
    Crystallization and preliminary X-ray analysis of SDR-type pyridoxal dehydrogenase from Mesorhizobium loti (2010), Acta Crystallogr. Sect. F, 66, 718-720.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mesorhizobium loti

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant selenomethionine-substituted enzyme, crystallized by the sitting-drop vapour-diffusion method using PEG 4000 as precipitant, in the presence of 0.45 mM NAD+. The crystals diffract to 2.9 A resolution and belong to the monoclinic space group P21 Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
x * 26000, SDS-PAGE Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti Q988B7
-
-
Mesorhizobium loti MAFF303099 Q988B7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Mesorhizobium loti

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
103.9
-
selenomethionine-substituted enzyme, 30°C, pH 9.2 Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxal + NAD+
-
Mesorhizobium loti 4-pyridoxolactone + NADH + H+
-
?
pyridoxal + NAD+
-
Mesorhizobium loti MAFF303099 4-pyridoxolactone + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Mesorhizobium loti

Synonyms

Synonyms Comment Organism
PLDH
-
Mesorhizobium loti

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mesorhizobium loti