General Stability | Organism |
---|---|
unstable without 2-mercaptoethanol, NAD+ stabilizes to a certain extent, the enzyme is unstable below pH 5.5 and above pH 10.0 even in the presence of NAD+ | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 1 mM, 50% inhibition after 4.6 min, half-inactivation time increases to 7.0 min in the presence of 20 mM D-pantoate | Pseudomonas fluorescens | |
iodacetic acid | 1 mM, 50% inhibition after 14 min, half-inactivation time increases to 29.0 min in the presence of 20 mM D-pantoate, 70% inhibition within 50 min at pH 7.2, 20 mM pantoate decreases inactivation from 70 to 30% | Pseudomonas fluorescens | |
p-chloromercuribenzoate | 0.1 mM, 85% inhibition after 15 min, 200 mM 2-mercaptoethanol restores 70% of enzyme activity within 10 min, 20 mM D-pantoate and 1 mM NAD+ prevent inactivation when added simultaneously | Pseudomonas fluorescens | |
Phenylglyoxal | 5.3 mM, 70% inhibition in borate buffer, 92% inhibition in NaHCO3 buffer within 10 min, complete inactivation after prolonged incubation with phenylglyoxal, NAD+ and D-pantoate increase half-inactivation time from 6 min without substrates to 40 and 17 min respectively | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
NAD+ | - |
Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-pantoate + NAD+ | Pseudomonas fluorescens | - |
(R)-4-dehydropantoate + NADH | - |
? | |
(R)-pantoate + NAD+ | Pseudomonas fluorescens UK1 | - |
(R)-4-dehydropantoate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
UK1 | - |
Pseudomonas fluorescens UK1 | - |
UK1 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, 5'-AMP-Sepharose 4B | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-pantoate + NAD+ | - |
Pseudomonas fluorescens | (R)-4-dehydropantoate + NADH | - |
? | |
(R)-pantoate + NAD+ | - |
Pseudomonas fluorescens UK1 | (R)-4-dehydropantoate + NADH | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas fluorescens |