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Literature summary for 1.1.1.103 extracted from

  • Motoyama, T.; Nakano, S.; Yamamoto, Y.; Tokiwa, H.; Asano, Y.; Ito, S.
    Product release mechanism associated with structural changes in monomeric L-threonine 3-dehydrogenase (2017), Biochemistry, 56, 5758-5770 .
    View publication on PubMed
Search for more literature for 1.1.1.103

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) uncultured archaeon

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor diffusion method at 4°C, crystal structures of the enzyme in apo, binary, and two ternary complexes (L-Ser- and L-Thr soaked forms) are determined at resolutions of 1.25-1.9 A uncultured archaeon

Protein Variants

Protein Variants Comment Organism
D179A mutation affects switching between the open and closed form of the enzyme. Catalytic efficiency (kcat/Km) of the mutant enzyme for L-Thr is 36fold lower than wild-type value uncultured archaeon
D179N mutation affects switching between the open and closed form of the enzyme. Catalytic efficiency (kcat/Km) of the mutant enzyme for L-Thr is 190fold lower than wild-type value uncultured archaeon
S111A mutation affects dehydrogenation of L-Thr directly. Catalytic efficiency (kcat/Km) of the mutant enzyme for L-Thr is 950fold lower than wild-type value uncultured archaeon
S74A mutation affects switching between the open and closed form of the enzyme. Catalytic efficiency (kcat/Km) of the mutant enzyme for L-Thr is 317fold lower than wild-type value uncultured archaeon
T177A mutation affects dehydrogenation of L-Thr directly. Catalytic efficiency (kcat/Km) of the mutant enzyme for L-Thr is 633fold lower than wild-type value uncultured archaeon
Y136F mutation affects dehydrogenation of L-Thr directly. Catalytic efficiency (kcat/Km) of the mutant enzyme for L-Thr is 127fold lower than wild-type value uncultured archaeon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 NAD+ 30°C, pH not specified in the publication, mutant enzyme S74A uncultured archaeon
0.0155
-
 NAD+ 30°C, pH not specified in the publication, mutant enzyme D179N uncultured archaeon
0.0196
-
 NAD+ 30°C, pH not specified in the publication, mutant enzyme S111A uncultured archaeon
0.0243
-
 NAD+ 30°C, pH not specified in the publication, mutant enzyme D179A uncultured archaeon
0.0257
-
 NAD+ 30°C, pH not specified in the publication, mutant enzyme T177A uncultured archaeon
0.0507
-
 NAD+ 30°C, pH not specified in the publication, wild-type enzyme uncultured archaeon
0.428
-
 NAD+ 30°C, pH not specified in the publication, mutant enzyme Y136F uncultured archaeon
23.7
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme T177A uncultured archaeon
25.3
-
 L-threonine 30°C, pH not specified in the publication, wild-type enzyme uncultured archaeon
40.6
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme Y136F uncultured archaeon
246
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme S111A uncultured archaeon

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ uncultured archaeon
-
 L-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
uncultured archaeon D6PBM7 MedDCM-OCT-S05-C57
-

Purification (Commentary)

Purification (Comment) Organism
-
 uncultured archaeon

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + NAD+
-
 uncultured archaeon L-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 uncultured archaeon

Synonyms

Synonyms Comment Organism
TDH
-
 uncultured archaeon

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme T177A uncultured archaeon
0.52
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme S111A uncultured archaeon
0.6
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme Y136F uncultured archaeon
48.8
-
 L-threonine 30°C, pH not specified in the publication, wild-type enzyme uncultured archaeon

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 uncultured archaeon

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme S111A uncultured archaeon
0.003
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme T177A uncultured archaeon
0.006
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme S74A uncultured archaeon
0.01
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme D179N uncultured archaeon
0.015
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme Y136F uncultured archaeon
0.053
-
 L-threonine 30°C, pH not specified in the publication, mutant enzyme D179A uncultured archaeon
1.9
-
 L-threonine 30°C, pH not specified in the publication, wild-type enzyme uncultured archaeon