KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADPH | - |
Aspergillus niger | |
0.13 | - |
NADP+ | - |
Aspergillus niger | |
17 | - |
L-xylulose | - |
Aspergillus niger | |
925 | - |
xylitol | - |
Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
8 * 32000, SDS-PAGE | Aspergillus niger |
250000 | - |
gel filtration | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-xylulose + NADPH + H+ | Aspergillus niger | - |
L-xylitol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33 | - |
xylitol oxidation, NADP+ | Aspergillus niger |
200 | - |
L-xylulose reduction, NADPH | Aspergillus niger |
Storage Stability | Organism |
---|---|
4°C, Tris-HCl 20 mM, pH 7.0, at leat several weeks | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sorbitol + NADP+ | - |
Aspergillus niger | ? | - |
? | |
L-xylulose + NADPH + H+ | - |
Aspergillus niger | L-xylitol + NADP+ | - |
r | |
additional information | minimal reductive activity also with D-xylulose, D-erythrose and dihydroxyacetone | Aspergillus niger | ? | - |
? | |
xylitol + NADP+ | - |
Aspergillus niger | L-xylulose + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 32000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
More | xylitol:NAD+ 2-oxidoreductase, EC 1.1.1.9, is termed as xylitol dehydrogenase | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction of L-xylulose | Aspergillus niger |
10 | 11 | oxidation of xylitol | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | reduction of L-xylulose | Aspergillus niger |
8 | 11.5 | oxidation of xylitol | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | determination of Ki of NADP+ and NADPH in competition with each other | Aspergillus niger | |
NAD+ | no activity with | Aspergillus niger | |
NADP+ | - |
Aspergillus niger | |
NADPH | preference for | Aspergillus niger |