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Literature summary for 1.1.1.1 extracted from
- Glutathione-dependent formaldehyde dehydrogenase homolog from Bacillus subtilis strain R5 is a propanol-preferring alcohol dehydrogenase (2017), Biochemistry, 82, 13-23 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-Codon-Plus(DE3)-RIL cells | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | significant inhibition at 10 mM | Bacillus subtilis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.12 | - |
ethanol | in the presence of 0.025 mM Cu2+, at pH 9.5 and 37°C | Bacillus subtilis | |
| 2.1 | - |
ethanol | in the presence of 0.025 mM Zn2+, at pH 9.5 and 37°C | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | the protein contains 1.3 mol of Cu2+. The highest activity, 22fold increase, is found in the protein produced in the presence of 0.025 mM Cu2+ | Bacillus subtilis | |
| Fe2+ | a 20fold increase in activity is found in the protein produced in the presence of 0.025 mM Fe2+ | Bacillus subtilis | |
| additional information | the presence or absence of metal ions (0.1 mM) in the reaction mixture does not affect the enzyme activity | Bacillus subtilis | |
| Zn2+ | the protein contains 1.5 mol of Zn2+. An increase in activity is found in the protein produced in the presence of 0.025 mM Zn2+ | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 164000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis R5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel chelate affinity column chromatography and Superdex 200 gel filtration | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-butanediol + NAD+ | 5% activity compared to 1-propanol | Bacillus subtilis | ? + NADH + H+ | - |
? | |
| 1-butanol + NAD+ | 34% activity compared to 1-propanol | Bacillus subtilis | butanal + NADH + H+ | - |
? | |
| 1-butanol + NAD+ | 34% activity compared to 1-propanol | Bacillus subtilis R5 | butanal + NADH + H+ | - |
? | |
| 1-hexanol + NAD+ | 7% activity compared to 1-propanol | Bacillus subtilis | hexanal + NADH + H+ | - |
? | |
| 1-hexanol + NAD+ | 7% activity compared to 1-propanol | Bacillus subtilis R5 | hexanal + NADH + H+ | - |
? | |
| ethanol + NAD+ | 63% activity compared to 1-propanol | Bacillus subtilis | acetaldehyde + NADH + H+ | - |
? | |
| ethanol + NAD+ | 63% activity compared to 1-propanol | Bacillus subtilis R5 | acetaldehyde + NADH + H+ | - |
? | |
| methanol + NAD+ | 16% activity compared to 1-propanol | Bacillus subtilis | formaldehyde + NADH + H+ | - |
? | |
| methanol + NAD+ | 16% activity compared to 1-propanol | Bacillus subtilis R5 | formaldehyde + NADH + H+ | - |
? | |
| additional information | no activity with isopropanol, 2,3-butanediol, isobutanol, isoamyl alcohol, cyclohexanol, acetone, cyclohexanone, acetophenone and alpha-tetralone | Bacillus subtilis | ? | - |
- |
|
| additional information | no activity with isopropanol, 2,3-butanediol, isobutanol, isoamyl alcohol, cyclohexanol, acetone, cyclohexanone, acetophenone and alpha-tetralone | Bacillus subtilis R5 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 42000, SDS-PAGE | Bacillus subtilis |
| homotetramer | 4 * 41208, calculated from amino acid sequence | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase | cf. EC 1.2.1.46 | Bacillus subtilis |
| GSH-FDH/ADH | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
temperature optimum for oxidation reaction in glycine NaOH buffer using ethanol as the substrate | Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 65 | more than 50% activity between 50 and 65°C | Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.78 | - |
ethanol | in the presence of 0.025 mM Zn2+, at pH 9.5 and 37°C | Bacillus subtilis | |
| 2.47 | - |
ethanol | in the presence of 0.025 mM Cu2+, at pH 9.5 and 37°C | Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
pH optimum for oxidation reaction in glycine NaOH buffer using ethanol as the substrate | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | dependent on | Bacillus subtilis | |
| NAD+ | - |
Bacillus subtilis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.85 | - |
ethanol | in the presence of 0.025 mM Zn2+, at pH 9.5 and 37°C | Bacillus subtilis | |
| 2.2 | - |
ethanol | in the presence of 0.025 mM Cu2+, at pH 9.5 and 37°C | Bacillus subtilis | |
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Release 2023.1 (January 2023)
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